Comparison of bone marrow tissue- and adipose tissue-derived mesenchymal stem cells in the treatment of sepsis in a murine model of lipopolysaccharide-induced sepsis

  • Authors:
    • Hao Ou
    • Shangping Zhao
    • Yue Peng
    • Xuefei Xiao
    • Qianlu Wang
    • Huaizeng Liu
    • Xianzhong Xiao
    • Mingshi Yang
  • View Affiliations

  • Published online on: August 30, 2016     https://doi.org/10.3892/mmr.2016.5694
  • Pages: 3862-3870
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Abstract

Mesenchymal stem cells (MSCs) have been reported to regulate the systemic inflammatory response and sepsis-induced immunologic injury pre-clinically. However, whether MSCs from different sources elicit identical effects remains to be elucidated. The present study compared the effect of bone marrow‑derived MSCs (BMSCs) and adipose tissue-derived MSCs (ADMSCs) in a murine model of lipopolysaccharide (LPS)‑induced sepsis. SPF BALB/c mice were induced with an injection of LPS (10 mg/kg; 1 mg/ml) via the tail vein. To compare the effect of MSCs on the septic mice, either saline, BMSCs or ADMSCs were injected via the tail vein 5 min following the administration of LPS. The survival rates and body temperatures of the mice were observed regularly up to 48 h. The serum levels of pro‑inflammatory cytokines, including tumour necrosis factor‑α, interleukin (IL)‑6 and IL‑8, anti‑inflammatory cytokines, including IL‑2, IL‑4 and IL‑10, and biochemical markers, including lactate, creatinine, alanine aminotransferase and aspertate aminotransferase, were analyzed at 6 h. The BMSCs and ADMSCs significantly reduced mortality rates, body‑temperature fluctuations, serum levels of biochemical markers and the majority of cytokines. However, the levels of IL‑8 in the BMSC and ADMSC groups were increased and decreased, respectively. These findings suggested that BMSCs and ADMSCs ameliorated sepsis-associated organ injury and mortality, and had a similar regulatory effect on pro‑ and anti‑inflammatory cytokines despite the different MSC sources. Therefore, BMSCs and ADMSCs may serve as novel treatment modalities for sepsis.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ou H, Zhao S, Peng Y, Xiao X, Wang Q, Liu H, Xiao X and Yang M: Comparison of bone marrow tissue- and adipose tissue-derived mesenchymal stem cells in the treatment of sepsis in a murine model of lipopolysaccharide-induced sepsis. Mol Med Rep 14: 3862-3870, 2016.
APA
Ou, H., Zhao, S., Peng, Y., Xiao, X., Wang, Q., Liu, H. ... Yang, M. (2016). Comparison of bone marrow tissue- and adipose tissue-derived mesenchymal stem cells in the treatment of sepsis in a murine model of lipopolysaccharide-induced sepsis. Molecular Medicine Reports, 14, 3862-3870. https://doi.org/10.3892/mmr.2016.5694
MLA
Ou, H., Zhao, S., Peng, Y., Xiao, X., Wang, Q., Liu, H., Xiao, X., Yang, M."Comparison of bone marrow tissue- and adipose tissue-derived mesenchymal stem cells in the treatment of sepsis in a murine model of lipopolysaccharide-induced sepsis". Molecular Medicine Reports 14.4 (2016): 3862-3870.
Chicago
Ou, H., Zhao, S., Peng, Y., Xiao, X., Wang, Q., Liu, H., Xiao, X., Yang, M."Comparison of bone marrow tissue- and adipose tissue-derived mesenchymal stem cells in the treatment of sepsis in a murine model of lipopolysaccharide-induced sepsis". Molecular Medicine Reports 14, no. 4 (2016): 3862-3870. https://doi.org/10.3892/mmr.2016.5694