Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon

  • Authors:
    • Ji Eun Kim
    • Eun Kyoung Koh
    • Sung Hwa Song
    • Ji Eun Sung
    • Hyun Ah Lee
    • Hong Gu Lee
    • Young Whan Choi
    • Dae Youn Hwang
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  • Published online on: December 5, 2016     https://doi.org/10.3892/mmr.2016.5983
  • Pages: 431-441
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Abstract

The laxative effects of aqueous extract of Liriope platyphylla (AEtLP) on loperamide (Lop)‑induced constipation have been reported; however, the key compounds and the mechanism underlying these effects remain unclear. Therefore, the laxative effects of five candidates derived from L. platyphylla: Diosgenin (DG), 5-hydroxymethylfurfural (5-HMF), adenosine (AD), hydroxypropyl cellulose (HPC) and uridine (UD) were investigated by examining the alteration of G protein α (Gα) expression, protein kinase C (PKC) phosphorylation and inositol triphosphate (IP3) concentration levels in the 5-hydroxytryptamine (5‑HT; serotonin) receptor signaling pathway. Primary rat intestine smooth muscle cells (pRISMCs), intestinal epithelial cells (IEC)‑18 and B35 cells were cotreated with Lop and the five compounds in order to screen the candidates. AEtLP, prucalopride (PCP) and bisacodyl (BS) served as positive controls. In pRISMCs, Gα expression levels were recovered in the majority of candidate‑treated groups, whereas PKC phosphorylation recovery was observed only in the DG, 5‑HMF and AD treatment groups. In IEC‑18 cells, the AD treatment group mimicked the effects of PCP on PKC phosphorylation levels, whereas the DG, 5‑HMF, HPC and UD treatment groups mimicked the effects of AEtLP and BS. In B35 cells, a greater upregulation of PKC phosphorylation levels were observed in the UD treatment group compared with the PCP and BS treatment groups, whereas DG, 5‑HMF and AD treatment reduced the PKC phosphorylation levels to a greater extent than AEtLP treatment. However, effects similar to AEtLP, PCP and BS on Gα expression levels were not detected in any treatment groups in IEC‑18 and B35 cells. Furthermore, the level of IP3 was enhanced only in pRISMCs, in which all five candidates were effective, while the greatest concentration was observed in the UD treatment group. In conclusion, the results of the present study suggest that UD may be considered the compound with the greatest laxative activity, which may regulate the 5‑HT receptor signaling pathway.
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January-2017
Volume 15 Issue 1

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Spandidos Publications style
Kim JE, Koh EK, Song SH, Sung JE, Lee HA, Lee HG, Choi YW and Hwang DY: Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon. Mol Med Rep 15: 431-441, 2017.
APA
Kim, J.E., Koh, E.K., Song, S.H., Sung, J.E., Lee, H.A., Lee, H.G. ... Hwang, D.Y. (2017). Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon. Molecular Medicine Reports, 15, 431-441. https://doi.org/10.3892/mmr.2016.5983
MLA
Kim, J. E., Koh, E. K., Song, S. H., Sung, J. E., Lee, H. A., Lee, H. G., Choi, Y. W., Hwang, D. Y."Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon". Molecular Medicine Reports 15.1 (2017): 431-441.
Chicago
Kim, J. E., Koh, E. K., Song, S. H., Sung, J. E., Lee, H. A., Lee, H. G., Choi, Y. W., Hwang, D. Y."Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon". Molecular Medicine Reports 15, no. 1 (2017): 431-441. https://doi.org/10.3892/mmr.2016.5983