Mutational spectrum of CENP-B box and α-satellite DNA on chromosome 21 in Down syndrome children

  • Authors:
    • Qian Chen
    • Bin Tan
    • Jun‑Lin He
    • Xue‑Qing Liu
    • Xue‑Mei Chen
    • Ru‑Fei Gao
    • Jing Zhu
    • Ying‑Xiong Wang
    • Hong‑Bo Qi
  • View Affiliations

  • Published online on: February 24, 2017     https://doi.org/10.3892/mmr.2017.6247
  • Pages: 2313-2317
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Abstract

The centromere is responsible for the correct inheritance of eukaryotic chromosomes during cell division. Centromere protein B (CENP‑B) and its 17 base pair binding site (CENP‑B box), which appears at regular intervals in centromeric α-satellite DNA (α-satDNA), are important for the assembly of the centromere components. Therefore, it is conceivable that CENP-B box mutations may induce errors in cell division. However, the association between the deoxynucleotide alterations of the CENP‑B box and the extra chromosome 21 (Chr21) present in patients with Down syndrome (DS) remains to be elucidated. The mutational spectrum of the α‑satDNA, including 4 functional CENP‑B boxes in Chr21 from 127 DS and 100 healthy children were analyzed by direct sequencing. The de novo occurrences of mutations within CENP‑B boxes in patients with DS were excluded. The prevalence of 6 novel mutations (g.661delC, g.1035_1036insA, g.1076_1077insC, g.670T>G, g.1239A>T, g.1343T>C) and 3 single nucleotide polymorphisms (g.727C/T, g.863A/C, g.1264C/G) were not significantly different between DS and controls (P>0.05). However, g.525C/G (P=0.01), g.601T/C (P=0.00000002), g.1279A/G (P=0.002), g.1294C/T (P=0.0006) and g.1302 G/T (P=0.004) were significantly associated with the prevalence of DS (P<0.05). The results indicated that CENP‑B boxes are highly conserved in DS patients and may not be responsible for Chr21 nondisjunction events. However, α‑satDNA in Chr21 is variable and deoxynucleotide deletions, mutations and polymorphisms may act as potential molecular diagnostic markers of DS.
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April-2017
Volume 15 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen Q, Tan B, He JL, Liu XQ, Chen XM, Gao RF, Zhu J, Wang YX and Qi HB: Mutational spectrum of CENP-B box and α-satellite DNA on chromosome 21 in Down syndrome children. Mol Med Rep 15: 2313-2317, 2017.
APA
Chen, Q., Tan, B., He, J., Liu, X., Chen, X., Gao, R. ... Qi, H. (2017). Mutational spectrum of CENP-B box and α-satellite DNA on chromosome 21 in Down syndrome children. Molecular Medicine Reports, 15, 2313-2317. https://doi.org/10.3892/mmr.2017.6247
MLA
Chen, Q., Tan, B., He, J., Liu, X., Chen, X., Gao, R., Zhu, J., Wang, Y., Qi, H."Mutational spectrum of CENP-B box and α-satellite DNA on chromosome 21 in Down syndrome children". Molecular Medicine Reports 15.4 (2017): 2313-2317.
Chicago
Chen, Q., Tan, B., He, J., Liu, X., Chen, X., Gao, R., Zhu, J., Wang, Y., Qi, H."Mutational spectrum of CENP-B box and α-satellite DNA on chromosome 21 in Down syndrome children". Molecular Medicine Reports 15, no. 4 (2017): 2313-2317. https://doi.org/10.3892/mmr.2017.6247