Different distributions of M1 and M2 macrophages in a mouse model of laser-induced choroidal neovascularization

Zhou,Yedi; Yoshida,Shigeo; Kubo,Yuki; Yoshimura,Takeru; Kobayashi,Yoshiyuki; Nakama,Takahito; Yamaguchi,Muneo; Ishikawa,Keijiro; Oshima,Yuji; Ishibashi,Tatsuro

doi:10.3892/mmr.2017.6491

Different distributions of M1 and M2 macrophages in a mouse model of laser-induced choroidal neovascularization

Authors:
- Yedi Zhou
- Shigeo Yoshida
- Yuki Kubo
- Takeru Yoshimura
- Yoshiyuki Kobayashi
- Takahito Nakama
- Muneo Yamaguchi
- Keijiro Ishikawa
- Yuji Oshima
- Tatsuro Ishibashi
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Affiliations: Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812‑8582, Japan
Published online on: April 20, 2017 https://doi.org/10.3892/mmr.2017.6491
Pages: 3949-3956
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Choroidal neovascularization (CNV) is a serious complication of age‑related macular degeneration. The aim of the present study was to investigate the expression and distribution of M1 and M2 macrophages in a laser‑induced CNV adult mouse model. The mRNA expression levels of M1, M2 and pan macrophage markers, and macrophage‑associated angiogenic cytokines, were determined by reverse transcription‑quantitative polymerase chain reaction. Immunofluorescence studies were performed to determine the location of the macrophages. The expression levels of M1 macrophage markers increased to a greater extent compared with M2 markers in the retinal pigment epithelium (RPE)‑choroid complexes following laser photocoagulation. By contrast, the expression levels of M2 macrophage markers increased primarily in the retinas. Immunofluorescence studies revealed that the increased number of cluster of differentiation (CD)206‑positive cells were located primarily in the retina, whereas the CD80‑positive cells were located around the site of CNVs in the RPE‑choroid. In addition, the M1‑associated cytokines increased to a greater extent in the RPE‑choroid complexes, whereas the M2‑associated cytokines were highly expressed in the retinas. These findings indicate that M1 and M2 macrophage numbers increased following CNV; however, the locations were different in this mouse model of laser‑induced CNV. The results of the present study suggest that M1 macrophages have a more direct role in inhibiting the development of CNV.

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