Differences in cell death and cell cycle following fucoidan treatment in high-density HT-29 colon cancer cells

Kim,In‑Hye; Kwon,Mi‑Jin; Nam,Taek‑Jeong

doi:10.3892/mmr.2017.6520

Differences in cell death and cell cycle following fucoidan treatment in high-density HT-29 colon cancer cells

Authors:
- In‑Hye Kim
- Mi‑Jin Kwon
- Taek‑Jeong Nam
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Affiliations: Cell Biology Laboratory, Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea
Published online on: April 27, 2017 https://doi.org/10.3892/mmr.2017.6520
Pages: 4116-4122
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fucoidan, a sulfated polysaccharide present in marine brown seaweed, has been demonstrated to inhibit in vivo and in vitro growth of cells. The present study was conducted in HT‑29 human colon cancer cells cultured at a high density, and examined the potential underlying mechanisms by which fucoidan exerts its anti‑proliferative effects, which remain poorly understood. Fucoidan treatment of high‑density HT‑29 cells resulted in the inhibition of cell growth and increased apoptotic cell death. Flow cytometric analysis revealed that fucoidan treatment led to sub‑G1 phase cell cycle arrest. This was associated with decreased protein expression levels of Retinoblastoma protein and E2 factor protein. In conclusion, the results of the present study demonstrated that fucoidan possesses anticancer activity against high density HT‑29 cells by inhibiting cell growth and cell cycle progression.

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