CD74-immunoreactive activated M1 microglia are shown late in the gerbil hippocampal CA1 region following transient cerebral ischemia

Hwang,In  Koo; Park,Joon  Ha; Lee,Tae‑Kyeong; Kim,Dae  Won; Yoo,Ki‑Yeon; Ahn,Ji  Hyeon; Kim,Yang  Hee; Cho,Jun  Hwi; Kim,Young‑Myeong; Won,Moo‑Ho; Moon,Seung  Myung

doi:10.3892/mmr.2017.6525

CD74-immunoreactive activated M1 microglia are shown late in the gerbil hippocampal CA1 region following transient cerebral ischemia

Authors:
- In Koo Hwang
- Joon Ha Park
- Tae‑Kyeong Lee
- Dae Won Kim
- Ki‑Yeon Yoo
- Ji Hyeon Ahn
- Yang Hee Kim
- Jun Hwi Cho
- Young‑Myeong Kim
- Moo‑Ho Won
- Seung Myung Moon
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Affiliations: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Oral Anatomy, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong, Gyeonggi 18450, Republic of Korea
Published online on: April 28, 2017 https://doi.org/10.3892/mmr.2017.6525
Pages: 4148-4154
Copyright: © Hwang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Activated M1 microglia secrete proinflammatory cytokines into damaged brain areas. The present study examined activated M1 microglial morphology and expression in the hippocampal Cornu Ammonis (CA) 1 region, which is vulnerable to transient ischemia. Transient cerebral ischemia was performed for 5 min in gerbils, and neuronal death in the CA1 region following transient cerebral ischemia was confirmed using cresyl violet staining, neuronal nuclear antigen immunohistochemistry and Fluoro‑Jade B histofluorescent staining. In addition, CA1 regions were stained for cluster of differentiation (CD) 74, a marker for activated M1 microglia and a ligand for macrophage migration inhibitory factor In sham‑operated animals, no CD74 immunoreactivity was observed in the hippocampal CA1 region. CD74 immunoreactivity was not observed in the hippocampal CA1 region until 3 days post‑ischemic insult; however, elevated CD74 immunoreactivity was detected in the CA1 region from 5 days post‑ischemia. Double immunofluorescence staining for CD74 and ionized calcium‑binding adapter molecule 1, a marker for M1 microglial cells, confirmed the expression of CD74 on this microglial subtype. These results indicated that M1 microglia are activated late in the hippocampal CA1 region following ischemic stroke. Therefore, optimizing the timing of therapeutic intervention may reduce activated M1 microglial-induced neuronal damage.

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