Open Access

Isolation of rat Schwann cells based on cell sorting

  • Authors:
    • Mi Shen
    • Wei Tang
    • Zheng Cao
    • Xuemin Cao
    • Fei Ding
  • View Affiliations

  • Published online on: June 14, 2017     https://doi.org/10.3892/mmr.2017.6777
  • Pages: 1747-1752
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study presented a protocol that can be used to obtain rapidly a high purity of proliferating rat Schwann cells from freshly dissociated rat peripheral nerves. The sciatic nerves of newborn rats (1‑3 day old) were dissociated, and the Schwann cells (SCs) were purified using fluorescence‑activated cell sorting (FACS) based on the SC membrane‑specific expression of the low‑affinity nerve growth factor receptor, p75NGFR and oligodendrocyte marker 4. Following sorting, the cells were plated on poly‑l‑lysine‑coated dishes in SC culture medium containing DMEM with 10% FBS, 1% penicillin/streptomycin, 2 µM forskolin and 10 ng/ml HRG. The purified rat SCs were propagated for passaging until confluent. This protocol resulted in SC cultures, which were >98% pure. This FACS‑based protocol can be used to facilitate future investigations of general SC biology.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shen M, Tang W, Cao Z, Cao X and Ding F: Isolation of rat Schwann cells based on cell sorting. Mol Med Rep 16: 1747-1752, 2017
APA
Shen, M., Tang, W., Cao, Z., Cao, X., & Ding, F. (2017). Isolation of rat Schwann cells based on cell sorting. Molecular Medicine Reports, 16, 1747-1752. https://doi.org/10.3892/mmr.2017.6777
MLA
Shen, M., Tang, W., Cao, Z., Cao, X., Ding, F."Isolation of rat Schwann cells based on cell sorting". Molecular Medicine Reports 16.2 (2017): 1747-1752.
Chicago
Shen, M., Tang, W., Cao, Z., Cao, X., Ding, F."Isolation of rat Schwann cells based on cell sorting". Molecular Medicine Reports 16, no. 2 (2017): 1747-1752. https://doi.org/10.3892/mmr.2017.6777