Open Access

miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4

  • Authors:
    • Jian‑Jun Qu
    • Xiang‑Yang Qu
    • De‑Zhen Zhou
  • View Affiliations

  • Published online on: July 21, 2017     https://doi.org/10.3892/mmr.2017.7057
  • Pages: 3731-3736
  • Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) have been demonstrated to be important in the establishment and progression of colon cancer. However the underlying molecular mechanisms remain to be fully elucidated. Polypeptide N‑acetylgalactosaminyltransferase4 (GALNT4) participates in numerous cellular processes, including tumorigenesis. The present study used reverse transcription‑quantitative polymerase chain reaction and western blotting to investigate the expression levels of miR‑4262 and GALNT4 in tissues and cells. In addition, MTS and colony formation assays, and cell cycle analysis were performed to evaluate the effect of miR‑4262 on cell proliferation and the cell cycle. The findings demonstrated that miR‑4262 was a direct target of GALNT4 mRNA. Overexpression of miR‑4262 was demonstrated to decrease GALNT4 mRNA and protein expression levels, and thereby suppressed cell viability, growth and cell‑cycle progression in SW480 and SW620 colon cancer cells. In addition, knockdown of miR‑4262 significantly increased the cell viability, growth, and cell‑cycle progression of SW480 and SW620 cells. The expression level of miR‑4262 was observed to be downregulated as the expression of GALNT4 was upregulated in colon cancer tissues and cell lines. In conclusion, the results demonstrated that miR‑4262 may be involved in the development of colon cancer via targeting of GALNT4. The miR‑4262/GALNT4 axis may be a novel target for diagnosing and understanding the underlying molecular mechanism of colon cancer.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Qu JJ, Qu XY and Zhou DZ: miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4. Mol Med Rep 16: 3731-3736, 2017
APA
Qu, J., Qu, X., & Zhou, D. (2017). miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4. Molecular Medicine Reports, 16, 3731-3736. https://doi.org/10.3892/mmr.2017.7057
MLA
Qu, J., Qu, X., Zhou, D."miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4". Molecular Medicine Reports 16.4 (2017): 3731-3736.
Chicago
Qu, J., Qu, X., Zhou, D."miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4". Molecular Medicine Reports 16, no. 4 (2017): 3731-3736. https://doi.org/10.3892/mmr.2017.7057