LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells

  • Authors:
    • Yang Xu
    • Zhixin Liang
    • Chunsun Li
    • Zhen Yang
    • Liangan Chen
  • View Affiliations

  • Published online on: July 27, 2017     https://doi.org/10.3892/mmr.2017.7095
  • Pages: 4159-4164
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Abstract

To win the war against lung cancer, the molecular mechanisms underlying its oncogenesis and metastasis must be identified in order to develop novel diagnosis and treatment strategies. We previously identified a novel gene, namely lung cancer metastasis related protein 1 (LCMR1; GenBank accession no. AY148462), which was demonstrated to be overexpressed in non‑small‑cell lung cancer. LCMR1 expression was significantly associated with clinical stage. To further understand the mechanism of LCMR1 in lung cancer, the present study screened a cDNA library from the lung cancer cell line 95D for proteins interacting with LCMR1 by yeast two‑hybrid assay, and the protein DEK was identified. Co‑immunoprecipitation and glutathione S‑transferase pull‑down assays were performed to confirm the interaction between LCMR1 and DEK in vivo and in vitro. The results demonstrated that the interaction was mediated primarily by the N‑terminal region of DEK, suggesting that LCMR1 may be involved in the regulation of cell apoptosis. Using RNA interference, DEK and LCMR1 were demonstrated to cooperate in the inhibition of apoptosis in lung cancer cells, and this effect was associated with the induced myeloid leukemia protein cell differentiation protein 1 pathway. The present findings suggest that LCMR1 might serve as a potential molecular target for lung cancer therapy.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Y, Liang Z, Li C, Yang Z and Chen L: LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells. Mol Med Rep 16: 4159-4164, 2017
APA
Xu, Y., Liang, Z., Li, C., Yang, Z., & Chen, L. (2017). LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells. Molecular Medicine Reports, 16, 4159-4164. https://doi.org/10.3892/mmr.2017.7095
MLA
Xu, Y., Liang, Z., Li, C., Yang, Z., Chen, L."LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells". Molecular Medicine Reports 16.4 (2017): 4159-4164.
Chicago
Xu, Y., Liang, Z., Li, C., Yang, Z., Chen, L."LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells". Molecular Medicine Reports 16, no. 4 (2017): 4159-4164. https://doi.org/10.3892/mmr.2017.7095