Open Access

Grb2-associated binder 2 expression and its roles in uveal melanoma invasion

  • Authors:
    • Meiling Chen
    • Yuehua Li
    • Xiuning Sun
    • Baogang Zhang
    • Wei Li
    • Shuxiao Wang
    • Xuetao Zhu
    • Feng Li
    • Lihong Shi
  • View Affiliations

  • Published online on: August 3, 2017     https://doi.org/10.3892/mmr.2017.7151
  • Pages: 4577-4582
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Uveal melanoma (UM) is characterized by high metastasis and poor prognosis. A more improved understanding of the metastatic mechanism in UM cells is essential for the design of molecular therapy. Grb2‑associated binder 2 (Gab2) has been reported to serve important roles in the progression of various types of human cancer. However, the role of Gab2 in the migration and invasion of UM remains unclear. The present study sought to further assess the expression of Gab2 in UM and the role of Gab2 in the invasion of UM cells. Clinical UM tissue samples and UM cell lines were analyzed using western blot analysis for the expression of Gab2. RNA interference was used to investigate the effect of Gab2 on the migratory and invasive characteristics of UM cells in vitro. The expression levels of matrix metalloproteinase (MMP)2, MMP9 and fascin in Gab2‑knockdown, and control cells were also detected using western blot analysis. A total of 20 clinical UM samples and a subset of UM cell lines were investigated with uniformly high Gab2 expression. In the in vitro experiment, reduction of Gab2 using small interfering RNA inhibited the migration and invasion of UM cells by mediating MMPs, and fascin expression. These data suggest that Gab2 is a useful prognostic marker for UM and a novel therapeutic target for UM metastasis intervention.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen M, Li Y, Sun X, Zhang B, Li W, Wang S, Zhu X, Li F and Shi L: Grb2-associated binder 2 expression and its roles in uveal melanoma invasion. Mol Med Rep 16: 4577-4582, 2017
APA
Chen, M., Li, Y., Sun, X., Zhang, B., Li, W., Wang, S. ... Shi, L. (2017). Grb2-associated binder 2 expression and its roles in uveal melanoma invasion. Molecular Medicine Reports, 16, 4577-4582. https://doi.org/10.3892/mmr.2017.7151
MLA
Chen, M., Li, Y., Sun, X., Zhang, B., Li, W., Wang, S., Zhu, X., Li, F., Shi, L."Grb2-associated binder 2 expression and its roles in uveal melanoma invasion". Molecular Medicine Reports 16.4 (2017): 4577-4582.
Chicago
Chen, M., Li, Y., Sun, X., Zhang, B., Li, W., Wang, S., Zhu, X., Li, F., Shi, L."Grb2-associated binder 2 expression and its roles in uveal melanoma invasion". Molecular Medicine Reports 16, no. 4 (2017): 4577-4582. https://doi.org/10.3892/mmr.2017.7151