Open Access

Human hepatocyte growth factor inhibits early neointima formation in rabbit abdominal aortae following ultrasound-guided balloon injury

  • Authors:
    • Li Mei
    • Yu He
    • Hui Wang
    • Ying Jin
    • Shuai Wang
    • Chunxiang Jin
  • View Affiliations

  • Published online on: August 11, 2017     https://doi.org/10.3892/mmr.2017.7229
  • Pages:5203-5210
  • Copyright: © Mei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the effects of in vivo gene transfer of human hepatocyte growth factor (hHGF) on neointima formation in rabbit abdominal aortae following ultrasound‑guided balloon injury. New Zealand white rabbits were randomly divided into four groups: endothelium injury alone (EI), endothelium injury with control vector transfection (EI‑V), endothelium injury with hHGF transfection (EI‑HGF), and hHGF transfection alone without endothelium injury (HGF). Endothelial injury was established by scraping the abdominal aortic wall using a balloon catheter under the guidance of a transabdominal ultrasound. hHGF gene transfer was performed 7 days following injury. hHGF mRNA and protein expression levels were determined at 3, 7, 14 and 21 days following transfection. Neointima formation was assessed by histopathological analysis at 14 and 28 days following injury. hHGF mRNA and protein expression levels were detected in the target abdominal aortae in EI‑HGF and HGF groups with the greatest levels observed 3 days following transfection, and their levels dropped below detection limits at 21 days following transfection. hHGF was not detectable in the EI and EI‑V groups throughout the experiment. The neointimal area and the neointima to media ratio in the EI‑HGF group were significantly decreased compared with those in the EI or EI‑V group at 14 days following injury. However, no differences were observed at 28 days following injury. The present study demonstrated that in vivo hHGF gene transfer inhibits the early formation of neointima in balloon‑injured rabbit abdominal aortae.

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October 2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Mei, L., He, Y., Wang, H., Jin, Y., Wang, S., & Jin, C. (2017). Human hepatocyte growth factor inhibits early neointima formation in rabbit abdominal aortae following ultrasound-guided balloon injury. Molecular Medicine Reports, 16, 5203-5210. https://doi.org/10.3892/mmr.2017.7229
MLA
Mei, L., He, Y., Wang, H., Jin, Y., Wang, S., Jin, C."Human hepatocyte growth factor inhibits early neointima formation in rabbit abdominal aortae following ultrasound-guided balloon injury". Molecular Medicine Reports 16.4 (2017): 5203-5210.
Chicago
Mei, L., He, Y., Wang, H., Jin, Y., Wang, S., Jin, C."Human hepatocyte growth factor inhibits early neointima formation in rabbit abdominal aortae following ultrasound-guided balloon injury". Molecular Medicine Reports 16, no. 4 (2017): 5203-5210. https://doi.org/10.3892/mmr.2017.7229