Association of a reduction of G‑protein coupled receptor 30 expression and the pathogenesis of preeclampsia

Feng,Xiang; Zhou,Liyuan; Mao,Xun; Tong,Chao; Chen,Xuyang; Zhao,Diqi; Baker,Philip  N.; Xia,Yinyin; Zhang,Hua

doi:10.3892/mmr.2017.7341

Association of a reduction of G‑protein coupled receptor 30 expression and the pathogenesis of preeclampsia

Authors:
- Xiang Feng
- Liyuan Zhou
- Xun Mao
- Chao Tong
- Xuyang Chen
- Diqi Zhao
- Philip N. Baker
- Yinyin Xia
- Hua Zhang
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Affiliations: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Canada‑China‑New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, P.R. China
Published online on: August 23, 2017 https://doi.org/10.3892/mmr.2017.7341
Pages: 5997-6003
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Preeclampsia is a pregnancy‑specific disorder, which is a leading cause of maternal and perinatal mortality and morbidity. A lower increase of estrogen, compared with the increase in progesterone, is associated with pathogenesis of the disease during pregnancy. G‑protein‑coupled receptor 30 (GPR30) mediates the action of estrogen, however remains to be investigated in preeclampsia. The levels of GPR30 were measured in placentae from uncomplicated pregnancies and pregnancies complicated by preeclampsia using immunohistochemistry and western blotting. GPR30 expression was additionally measured in placental HTR8/SVneo cells following 17β‑estrogen (E2) treatment in normal or hypoxia‑reoxygenation conditions by western blotting. In addition, the outgrowth of HTR8/SVneo cells following E2 treatment in hypoxia‑reoxygenation conditions was measured. Levels of GPR30 were significantly reduced in placentae from women with preeclampsia as compared with uncomplicated pregnancies. Treatment with E2 significantly increased the expression of GPR30 in HTR8/SVneo cells, in normal and hypoxia‑reoxygenation conditions. Furthermore, treatment with E2 increased the outgrowth of HTR8/SVneo cells in hypoxia‑reoxygenation conditions. The present study demonstrated lowered placental expression of GPR30 in preeclampsia. Estrogen treatment increases GPR30 expression in extravillous trophoblast and GPR30 may be involved in extravillous trophoblast invasion.

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