Open Access

Aconitine induces apoptosis in H9c2 cardiac cells via mitochondria‑mediated pathway

  • Authors:
    • Xiangting Gao
    • Xincai Zhang
    • Jun Hu
    • Xuehua Xu
    • Yuanyi Zuo
    • Yun Wang
    • Jingfeng Ding
    • Hongfei Xu
    • Shaohua Zhu
  • View Affiliations

  • Published online on: October 26, 2017     https://doi.org/10.3892/mmr.2017.7894
  • Pages: 284-292
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aconitine, a diterpenoid alkaloids derived from Aconitum plants, is widely employed to treat various diseases. The aim of the present study was to investigate the apoptotic effect of aconitine in H9c2 cardiac cells. H9c2 cell apoptosis induced by aconitine was detected by a Cell Counting kit‑8 assay, DAPI staining, Annexin V‑FITC/propidium iodide double staining and western blotting. The effects of aconitine on reactive oxygen species levels and mitochondrial membrane potential were confirmed by fluorescence microscopy and flow cytometry. In addition, ATP contents were determined using a ATP‑dependent bioluminescence assay kit. The levels of peroxisome proliferator activated receptor γ co‑activator 1α (PGC‑1α) expression and apoptosis‑associated proteins including Caspase‑3, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and Cytochrome c were also assessed. Taken together, the results indicated that aconitine may inhibit cell viability, decrease PGC‑1α expression, induce mitochondrial dysfunctions, upregulate Cytochrome c, Bax and Caspase‑3, and downregulate Bcl‑2, suggesting that aconitine may induce apoptosis through mitochondria‑mediated signaling pathways in H9c2 cells.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Gao X, Zhang X, Hu J, Xu X, Zuo Y, Wang Y, Ding J, Xu H and Zhu S: Aconitine induces apoptosis in H9c2 cardiac cells via mitochondria‑mediated pathway. Mol Med Rep 17: 284-292, 2018.
APA
Gao, X., Zhang, X., Hu, J., Xu, X., Zuo, Y., Wang, Y. ... Zhu, S. (2018). Aconitine induces apoptosis in H9c2 cardiac cells via mitochondria‑mediated pathway. Molecular Medicine Reports, 17, 284-292. https://doi.org/10.3892/mmr.2017.7894
MLA
Gao, X., Zhang, X., Hu, J., Xu, X., Zuo, Y., Wang, Y., Ding, J., Xu, H., Zhu, S."Aconitine induces apoptosis in H9c2 cardiac cells via mitochondria‑mediated pathway". Molecular Medicine Reports 17.1 (2018): 284-292.
Chicago
Gao, X., Zhang, X., Hu, J., Xu, X., Zuo, Y., Wang, Y., Ding, J., Xu, H., Zhu, S."Aconitine induces apoptosis in H9c2 cardiac cells via mitochondria‑mediated pathway". Molecular Medicine Reports 17, no. 1 (2018): 284-292. https://doi.org/10.3892/mmr.2017.7894