The antibacterial effect of topical ozone on the treatment of MRSA skin infection

Song,Mingsheng; Zeng,Qinghai; Xiang,Yaping; Gao,Lihua; Huang,Jian; Huang,Jinhua; Wu,Kathy; Lu,Jianyun

doi:10.3892/mmr.2017.8148

The antibacterial effect of topical ozone on the treatment of MRSA skin infection

Authors:
- Mingsheng Song
- Qinghai Zeng
- Yaping Xiang
- Lihua Gao
- Jian Huang
- Jinhua Huang
- Kathy Wu
- Jianyun Lu
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Affiliations: Department of Laboratory Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
Published online on: November 24, 2017 https://doi.org/10.3892/mmr.2017.8148
Pages: 2449-2455
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Skin can be infected by many types of microorganisms, most commonly by gram‑positive strains of Staphylococcus and Streptococcus spp. Treatment of Staphylococcus aureus (S. aureus) infections, particularly that of methicillin resistant Staphylococcus aureus (MRSA), is a challenge in clinical practice. Ozone therapy has proven to be one of the strongest antiseptics against the majority of microorganisms involved in skin infections. The purpose of the present study was to evaluate the microbicidal effects of topical ozone therapy on S. aureus and MRSA, and determine the clinical efficacy of ozone therapy on patients with MRSA skin infection. Microbicidal effects of ozonated oil and ozonated water were determined by plating and Kirby Bauer methods. Clinical efficacy and safety of topical ozone were evaluated in two cases with skin MRSA infection. The killing rates of ozonated oil for S. aureus and MRSA were greater when compared with the control oil group. Almost 100% of S. aureus were eliminated by ozonated oil following 5 min. Almost 100% MRSA were eliminated by ozonated oil following 15 min. In addition, 100% S. aureus and 100% MRSA were eliminated by ozonated water in 1 min. The inhibition zone diameters of ozonated oil for S. aureus and MRSA were 17 and 13 mm, respectively, which were significantly larger than the control group. Both cases of skin MRSA infection were completely healed with ozone therapy. In conclusion, ozone therapy is a potential treatment for S. aureus and MRSA skin infection as it has great efficacy, few side effects and low‑costs.

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