Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes

  • Authors:
    • Ken Kataoka
    • Natsumi Maehara
    • Yuki Ayabe
    • Hitoshi Murata
    • Nam‑Ho Huh
    • Masakiyo Sakaguchi
  • View Affiliations

  • Published online on: March 5, 2018     https://doi.org/10.3892/mmr.2018.8676
  • Pages: 6661-6666
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Abstract

Our previous studies revealed that REIC/Dkk-3 was expressed various tissues, including skin keratinocytes. The aim of the present study was to identify the factors that regulate the expression of the dickkopf Wnt signaling pathway inhibitor 3 (REIC/Dkk‑3) tumor suppressor gene in normal human skin keratinocytes (NHKs). Several growth factors and cytokines that have previously been reported to be involved in the growth and differentiation of keratinocytes were screened as potential regulators. Western blot analysis was performed using protein from NHKs cultured with/without various factors including the epidermal growth factor, tumor necrosis factor‑α, transforming growth factor‑β, interleukin (IL)‑1F9, IL‑6, IL‑8 and Ca2+. The results indicated that only TNF‑α downregulated REIC/Dkk‑3 expression in NHKs. Subsequently, TNF‑α was confirmed to reduce the expression levels of REIC/Dkk‑3 in mouse skin tissue and hair culture models. TNF‑α‑mediated downregulation of REIC/Dkk‑3 expression in NHKs was abrogated by the addition of a TNF‑α‑specific antibody. In conclusion, the results indicate that TNF‑α downregulates REIC/Dkk‑3 expression in normal skin keratinocytes.
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May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kataoka K, Maehara N, Ayabe Y, Murata H, Huh NH and Sakaguchi M: Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Mol Med Rep 17: 6661-6666, 2018
APA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., & Sakaguchi, M. (2018). Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Molecular Medicine Reports, 17, 6661-6666. https://doi.org/10.3892/mmr.2018.8676
MLA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17.5 (2018): 6661-6666.
Chicago
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17, no. 5 (2018): 6661-6666. https://doi.org/10.3892/mmr.2018.8676