Impact of epigallocatechin‑3‑gallate on expression of nuclear factor erythroid 2‑related factor 2 and γ‑glutamyl cysteine synthetase genes in oxidative stress‑induced mouse renal tubular epithelial cells

Du,Xuanyi; Yu,Jinfeng; Sun,Xiaohan; Qu,Shaochuan; Zhang,Haitao; Hu,Mengying; Yang,Shufen; Zhou,Ping

doi:10.3892/mmr.2018.8798

Impact of epigallocatechin‑3‑gallate on expression of nuclear factor erythroid 2‑related factor 2 and γ‑glutamyl cysteine synthetase genes in oxidative stress‑induced mouse renal tubular epithelial cells

Authors:
- Xuanyi Du
- Jinfeng Yu
- Xiaohan Sun
- Shaochuan Qu
- Haitao Zhang
- Mengying Hu
- Shufen Yang
- Ping Zhou
View Affiliations

Affiliations: Department of Nephrology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Pediatrics, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Pediatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
Published online on: March 28, 2018 https://doi.org/10.3892/mmr.2018.8798
Pages: 7952-7958

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the antioxidant response mechanism of epigallocatechin‑3‑gallate (EGCG) in H2O2‑induced mouse renal tubular epithelial cells (MRTECs). The cultured MRTECs were divided into normal, H2O2 (control) and EGCG treatment groups. The MTT assay was used to assess cell viability, and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), immunocytochemical and western blot analyses were performed to detect the expression of nuclear factor erythroid 2‑related factor 2 (Nrf2) and γ‑glutamyl cysteine synthetase (γ‑GCS). EGCG was able to mitigate H2O2‑mediated cell damage. The RT‑qPCR results demonstrated that EGCG was able to upregulate the gene expression of Nrf2 and γ‑GCS in MRTECs in a dose‑dependent manner. The immunocytochemistry and western blot analyses demonstrated that EGCG was able to increase the protein expression of Nrf2 and γ‑GCS in MRTECs in a dose‑dependent manner. Oxidative stress may lead to a decrease in the viability of MRTECs, while EGCG was able to promote the expression of Nrf2 and γ‑GCS in MRTECs, thereby improving the antioxidant capacity of the cells and promoting the repair of oxidative stress injury.

View Figures

View References

1	Rani V, Deep G, Singh RK, Palle K and Yadav UC: Oxidative stress and metabolic disorders: Pathogenesis and therapeutic strategies. Life Sci. 148:183–193. 2016. View Article : Google Scholar : PubMed/NCBI
2	Tamay-Cach F, Quintana-Pérez JC, Trujillo-Ferrara JG, Cuevas-Hernández RI, Del Valle-Mondragón L, García-Trejo EM and Arellano-Mendoza MG: A review of the impact of oxidative stress and some antioxidant therapies on renal damage. Ren Fail. 38:171–175. 2016. View Article : Google Scholar : PubMed/NCBI
3	Lindblom R, Higgins G, Coughlan M and de Haan JB: Targeting mitochondria and reactive oxygen species-driven pathogenesis in diabetic nephropathy. Rev Diabet Stud. 12:134–156. 2015. View Article : Google Scholar : PubMed/NCBI
4	Cervellati F, Cervellati C, Romani A, Cremonini E, Sticozzi C, Belmonte G, Pessina F and Valacchi G: Hypoxia induces cell damage via oxidative stress in retinal epithelial cells. Free Radic Res. 48:303–312. 2014. View Article : Google Scholar : PubMed/NCBI
5	Costa JG, Saraiva N, Guerreiro PS, Louro H, Silva MJ, Miranda JP, Castro M, Batinic-Haberle I, Fernandes AS and Oliveira NG: Ochratoxin A-induced cytotoxicity, genotoxicity and reactive oxygen species in kidney cells: An integrative approach of complementary endpoints. Food Chem Toxicol. 87:65–76. 2016. View Article : Google Scholar : PubMed/NCBI
6	Choi K, Ortega MT, Jeffery B, Riviere JE and Monteiro-Riviere NA: Oxidative stress response in canine in vitro liver, kidney and intestinal models with seven potential dietary ingredients. Toxicol Lett. 241:49–59. 2016. View Article : Google Scholar : PubMed/NCBI
7	Cho YE, Kim HS, Lai C, Stanfill A and Cashion A: Oxidative stress is associated with weight gain in recipients at 12-months following kidney transplantation. Clin Biochem. 49:237–242. 2016. View Article : Google Scholar : PubMed/NCBI
8	Liu Y, Qiu J, Wang Z, You W, Wu L, Ji C and Chen G: Dimethylfumarate alleviates early brain injury and secondary cognitive deficits after experimental subarachnoid hemorrhage via activation of Keap1-Nrf2-ARE system. J Neurosurg. 123:915–923. 2015. View Article : Google Scholar : PubMed/NCBI
9	Tanaka Y, Kume S, Araki H, Nakazawa J, Chin-Kanasaki M, Araki S, Nakagawa F, Koya D, Haneda M, Maegawa H and Uzu T: 1-Methylnicotinamide ameliorates lipotoxicity-induced oxidative stress and cell death in kidney proximal tubular cells. Free Radic Biol Med. 89:831–841. 2015. View Article : Google Scholar : PubMed/NCBI
10	Andrich K and Bieschke J: The Effect of (−)-Epigallo-catechin-(3)-gallate on amyloidogenic proteins suggests a common mechanism. Adv Exp Med Biol. 863:139–161. 2015. View Article : Google Scholar : PubMed/NCBI
11	Navarro E, Serrano-Heras G, Castaño MJ and Solera J: Real-time PCR detection chemistry. Clin Chim Acta. 439:231–250. 2015. View Article : Google Scholar : PubMed/NCBI
12	Soung HS, Wang MH, Tseng HC, Fanga HW and Chang KC: (−)Epigallocatechin-3-gallate decreases the stress-induced impairment of learning and memory in rats. Neurosci Lett. 602:27–32. 2015. View Article : Google Scholar : PubMed/NCBI
13	Cai S, Zhong Y, Li Y, Huang J, Zhang J, Luo G and Liu Z: Blockade of the formation of insoluble ubiquitinated protein aggregates by EGCG3′ Me in the alloxan-induced diabetic kidney. PLoS One. 8:e756872013. View Article : Google Scholar : PubMed/NCBI
14	Hyung SJ, DeToma AS, Brender JR, Lee S, Vivekanandan S, Kochi A, Choi JS, Ramamoorthy A, Ruotolo BT and Lim MH: Insights into antiamyloidogenic properties of the green tea extract (−)-epigallocatechin-3-gallate toward metal-associated amyloid-β species. Proc Natl Acad Sci USA. 110:3743–3748. 2013. View Article : Google Scholar : PubMed/NCBI
15	Wang Y, Liu N, Bian X, Sun G, Du F, Wang B, Su X and Li D: Epigallocatechin-3-gallate reduces tubular cell apoptosis in mice with ureteral obstruction. J Surg Res. 197:145–154. 2015. View Article : Google Scholar : PubMed/NCBI
16	Zhao CG, Zhou P and Wu YB: Impact and significance of EGCG on Smad, ERK, and β-catenin pathways in transdifferentiation of renal tubular epithelial cells. Genet Mol Res. 14:2551–2560. 2015. View Article : Google Scholar : PubMed/NCBI
17	Tao L, Forester SC and Lambert JD: The role of the mitochondrial oxidative stress in the cytotoxic effects of the green tea catechin, (−)-epigallocatechin-3-gallate, in oral cells. Mol Nutr Food Res. 58:665–676. 2014. View Article : Google Scholar : PubMed/NCBI
18	Zhong RZ, Fang Y, Qin GX, Li HY and Zhou DW: Tea catechins protect goat skeletal muscle against H₂O₂-induced oxidative stress by modulating expression of phase 2 antioxidant enzymes. J Agric Food Chem. 63:7921–7928. 2015. View Article : Google Scholar : PubMed/NCBI
19	Zhai W, Zheng J, Yao X, Peng B, Liu M, Huang J, Wang G and Xu Y: Catechin prevents the calcium oxalate monohydrate induced renal calcium crystallization in NRK-52E cells and the ethylene glycol induced renal stone formation in rat. BMC Complement Altern Med. 13:2282013. View Article : Google Scholar : PubMed/NCBI
20	Ding X, Wang D, Li L and Ma H: Dehydroepiandrosterone ameliorates H₂O₂-induced Leydig cells oxidation damage and apoptosis through inhibition of ROS production and activation of PI3K/Akt pathways. Int J Biochem Cell Biol. 70:126–139. 2016. View Article : Google Scholar : PubMed/NCBI
21	Schultz MA, Abdel-Mageed AB and Mondal D: The Nrf1 and Nrf2 balance in oxidative stress regulation and androgen signaling in prostate cancer cells. Cancers (Basel). 2:1354–1378. 2010. View Article : Google Scholar : PubMed/NCBI
22	Furnari MA, Saw CL, Kong AN and Wagner GC: Altered behavioral development in Nrf2 knockout mice following early postnatal exposure to valproic acid. Brain Res Bul. 109:132–142. 2014. View Article : Google Scholar
23	Khoi PN, Park JS, Kim JH, Xia Y, Kim NH, Kim KK and Jung YD: (−)-Epigallocatechin-3-gallate blocks nicotine-induced matrix metalloproteinase-9 expression and invasiveness via suppression of NF-κB and AP-1 in endothelial cells. Int J Oncol. 43:868–876. 2013. View Article : Google Scholar : PubMed/NCBI
24	Park SY, Jeong YJ, Kim SH, Jung JY and Kim WJ: Epigallocatechin gallate protects against nitric oxide-induced apoptosis via scavenging ROS and modulating the Bcl-2 family in human dental pulp cells. J Toxicol Sci. 38:371–378. 2013. View Article : Google Scholar : PubMed/NCBI
25	Feng B, Fang Y and Wei SM: Effect and mechanism of epigallocatechin-3-gallate (EGCG). against the hydrogen peroxide-induced oxidative damage in human dermal fibroblasts. J Cosmet Sci. 64:35–44. 2013.PubMed/NCBI
26	Toniolo A, Buccellati C, Pinna C, Gaion RM, Sala A and Bolego C: Cyclooxygenase-1 and prostacyclin production by endothelial cells in the presence of mild oxidative stress. PLoS One. 8:e566832013. View Article : Google Scholar : PubMed/NCBI
27	Deshmukh P, Unni S, Krishnappa G and Padmanabhan B: The Keap1–Nrf2 pathway: Promising therapeutic target to counteract ROS-mediated damage in cancers and neurodegenerative diseases. Biophys Rev. 9:41–56. 2017. View Article : Google Scholar : PubMed/NCBI
28	Magesh S, Chen Y and Hu L: Small molecule modulators of Keap1-Nrf2-ARE pathway as potential preventive and therapeutic agents. Med Res Rev. 32:687–726. 2012. View Article : Google Scholar : PubMed/NCBI
29	Mitsuishi Y, Motohashi H and Yamamoto M: The Keap1–Nrf2 system in cancers: Stress response and anabolic metabolism. Front Oncol. 2:2002012. View Article : Google Scholar : PubMed/NCBI
30	Sandberg M, Patil J, D'Angelo B, Weber SG and Mallard C: NRF2-regulation in brain health and disease: Implication of cerebral inflammation. Neuropharmacology. 79:298–306. 2014. View Article : Google Scholar : PubMed/NCBI
31	Tebay LE, Robertson H, Durant ST, Vitale SR, Penning TM, Dinkova-Kostova AT and Hayes JD: Mechanisms of activation of the transcription factor Nrf2 by redox stressors, nutrient cues, and energy status and the pathways through which it attenuates degenerative disease. Free Radic Biol Med. 88:108–146. 2015. View Article : Google Scholar : PubMed/NCBI
32	O'Brien S and Kay NE: Maintenance therapy for B-chronic lymphocytic leukemia. Clin Adv Hematol Oncol. 9:22–31. 2011.PubMed/NCBI
33	Muthusamy VR, Kannan S, Sadhaasivam K, Gounder SS, Davidson CJ, Boeheme C, Hoidal JR, Wang L and Rajasekaran NS: Acute exercise stress activates Nrf2/ARE signaling and promotes antioxidant mechanisms in the myocardium. Free Radic Biol Med. 52:366–376. 2012. View Article : Google Scholar : PubMed/NCBI
34	Ma Q: Role of Nrf2 in oxidative stress and toxicity. Annu Rev Pharmacol Toxicol. 53:401–426. 2013. View Article : Google Scholar : PubMed/NCBI