Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells

Shen,Gui‑Nan; Liu,Lei; Feng,Li; Jin,Yu; Jin,Mei‑Hua; Han,Ying‑Hao; Jin,Cheng‑Hao; Jin,Yong‑Zhe; Lee,Dong‑Soek; Kwon,Tae Ho; Cui,Yu‑Dong; Sun,Hu‑Nan

doi:10.3892/mmr.2018.8826

Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells

Authors:
- Gui‑Nan Shen
- Lei Liu
- Li Feng
- Yu Jin
- Mei‑Hua Jin
- Ying‑Hao Han
- Cheng‑Hao Jin
- Yong‑Zhe Jin
- Dong‑Soek Lee
- Tae Ho Kwon
- Yu‑Dong Cui
- Hu‑Nan Sun
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Affiliations: Department of Disease Model Animal Research Center, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China, Pharmaron Beijing Co., Ltd., Beijing 100176, P.R. China, Laboratory of Anatomy and Histology, Yanbian University Health Science Center, Yanji, Jilin 133000, P.R. China, Laboratory of Molecular Neurobiology, School of Life Sciences, KNU Creative Bio Research Group (BK21 Plus Project), Kyungpook National University, Daegu 41566, Republic of Korea, Laboratory of Animal Genetic Engineering and Stem Cell Biology, Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, Jeju 63243, Republic of Korea
Published online on: March 29, 2018 https://doi.org/10.3892/mmr.2018.8826
Pages: 7827-7834

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Abstract

High concentrations of glutamate may mediate neuronal cell apoptosis by increasing intracellular reactive oxygen species (ROS) levels. Peroxiredoxin V (Prx V), a member of the Prx family, serves crucial roles in protecting cells from oxidative stress. The present study investigated the regulatory effect of Prx V on glutamate‑induced effects on viability and apoptosis in HT22 cells. Western blotting was used for protein expression analysis and Annexin V/PI staining and flow cytometry for determination of apoptosis. The results demonstrated that glutamate may ROS‑dependently increase HT22 cell apoptosis and upregulate Prx V protein levels. Furthermore, knockdown of Prx V protein expression with a lentivirus significantly enhanced HT22 cell apoptosis mediated by glutamate, which was reversed by inhibition of ROS with N‑acetyl‑L‑cysteine. Inhibiting the extracellular signal‑regulated kinase (ERK) signaling pathway with PD98059, a specific inhibitor for ERK phosphorylation, markedly decreased glutamate‑induced HT22 cell apoptosis in Prx V knockdown cells, indicating the potential involvement of ERK signaling in glutamate‑induced HT22 cell apoptosis. In addition, an increase in nuclear apoptosis‑inducing factor was observed in Prx V knockdown HT22 cells following glutamate treatment, compared with mock cells, whereas no differences in B‑cell lymphoma‑2 and cleaved‑caspase‑3 protein expression levels were observed between mock and Prx V knockdown cells. The results of the present study indicated that Prx V may have potential as a therapeutic molecular target for glutamate‑induced neuronal cell death and provide novel insight into the role of Prx V in oxidative‑stress induced neuronal cell death.

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