miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2

  • Authors:
    • Mingjun Lu
    • Lingling Xu
    • Min Wang
    • Tao Guo
    • Fuquan Luo
    • Nan Su
    • Shanghui Yi
    • Tao Chen
  • View Affiliations

  • Published online on: April 19, 2018     https://doi.org/10.3892/mmr.2018.8903
  • Pages: 8502-8509
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Abstract

To investigate the role of microRNA (miR)‑149 in the cardiac differentiation of mouse bone marrow mesenchymal stem cells (MSCs) in vitro, MSCs were infected with a lentivirus overexpressing miR‑149 and the effect on cardiac differentiation was determined. The quantitative polymerase chain reaction results demonstrated that miR‑149 promoted the expression of cardiac‑specific markers in MSCs. Western blotting and a luciferase activity assay demonstrated that disabled homolog 2 (Dab2) was a direct target of miR‑149. Dab2 ectopic expression and Wnt/β‑catenin signaling pathway inhibition was able to reverse the increased expression of cardiac‑specific markers induced by miR‑149. In conclusion, miR‑149 was able to target Dab2 and promote the cardiac differentiation of mouse MSCs in vitro, which depended upon the Wnt/β‑catenin signaling pathway.
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June-2018
Volume 17 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu M, Xu L, Wang M, Guo T, Luo F, Su N, Yi S and Chen T: miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2. Mol Med Rep 17: 8502-8509, 2018.
APA
Lu, M., Xu, L., Wang, M., Guo, T., Luo, F., Su, N. ... Chen, T. (2018). miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2. Molecular Medicine Reports, 17, 8502-8509. https://doi.org/10.3892/mmr.2018.8903
MLA
Lu, M., Xu, L., Wang, M., Guo, T., Luo, F., Su, N., Yi, S., Chen, T."miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2". Molecular Medicine Reports 17.6 (2018): 8502-8509.
Chicago
Lu, M., Xu, L., Wang, M., Guo, T., Luo, F., Su, N., Yi, S., Chen, T."miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2". Molecular Medicine Reports 17, no. 6 (2018): 8502-8509. https://doi.org/10.3892/mmr.2018.8903