Obtusifolin inhibits high glucose‑induced mitochondrial apoptosis in human umbilical vein endothelial cells

  • Authors:
    • Yu Tang
    • Zhi‑Ying Zhong
    • Yan‑Feng Liu
    • Gou‑Tai Sheng
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  • Published online on: July 5, 2018     https://doi.org/10.3892/mmr.2018.9251
  • Pages: 3011-3019
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Abstract

DM is often accompanied by macrovascular complications. Obtusifolin, which is an anthraquinone‑based compound with antioxidant activity, is obtained from the seeds of Cassia obtusifolia. In this study, the potential effect of obtusifolin was investigated in human umbilical vein endothelial cells. The results from flow cytometry analysis revealed that pretreatment with obtusifolin depressed the production of cellular reactive oxygen species that was induced by high glucose content. Moreover, the results showed that pretreatment with obtusifolin reduced the level of malondialdehyde, as well as recovered the activities of mitochondrial complex I/III, catalase and superoxide dismutase. Furthermore, flow cytometry analysis also revealed that mitochondrial membrane potential and cell apoptosis were recovered, and inhibited by obtusifolin, respectively. The expression of X chromosome‑linked IAP was upregulated, whereas the expressions of poly ADP‑ribose polymerase and cysteinyl aspartate specific proteinase‑3/9 were downregulated by the pretreatment with obtusifolin. Notably, the western blot analyses showed that the release of Omi/HtrA2 into the cytosol was prevented by the pretreatment with obtusifolin. Conclusively, it was suggested that obtusifolin may provide protection against mitochondrial apoptosis largely through inhibition of the release of Omi/HtrA2 from mitochondria into cytosol.
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September-2018
Volume 18 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tang Y, Zhong ZY, Liu YF and Sheng GT: Obtusifolin inhibits high glucose‑induced mitochondrial apoptosis in human umbilical vein endothelial cells. Mol Med Rep 18: 3011-3019, 2018
APA
Tang, Y., Zhong, Z., Liu, Y., & Sheng, G. (2018). Obtusifolin inhibits high glucose‑induced mitochondrial apoptosis in human umbilical vein endothelial cells. Molecular Medicine Reports, 18, 3011-3019. https://doi.org/10.3892/mmr.2018.9251
MLA
Tang, Y., Zhong, Z., Liu, Y., Sheng, G."Obtusifolin inhibits high glucose‑induced mitochondrial apoptosis in human umbilical vein endothelial cells". Molecular Medicine Reports 18.3 (2018): 3011-3019.
Chicago
Tang, Y., Zhong, Z., Liu, Y., Sheng, G."Obtusifolin inhibits high glucose‑induced mitochondrial apoptosis in human umbilical vein endothelial cells". Molecular Medicine Reports 18, no. 3 (2018): 3011-3019. https://doi.org/10.3892/mmr.2018.9251