CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44

Fu,Zhichao; Zhang,Pei; Luo,Huachun; Huang,Huijuan; Wang,Fengmei

doi:10.3892/mmr.2018.9554

CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44

Authors:
- Zhichao Fu
- Pei Zhang
- Huachun Luo
- Huijuan Huang
- Fengmei Wang
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Affiliations: Department of Radiation Oncology, Dongfang Hospital, Xiamen University, Fuzhou, Fujian 350025, P.R. China, Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, P.R. China, Department of Radiation Oncology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, Fujian 350025, P.R. China, Department of Gynaecology and Obstetrics, Dongfang Hospital, Xiamen University, Fuzhou, Fujian 350025, P.R. China
Published online on: October 12, 2018 https://doi.org/10.3892/mmr.2018.9554
Pages: 5101-5108

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Abstract

The aim of the present study was to investigate the regulation of stromal cell‑derived factor 1 (CXCL12) in the radioresistance of cervical cancer, which was upregulated in tumors in our previous study. A CCK‑8 assay was used to detect cell viability. Flow cytometry was used to measure cell apoptosis and the expression levels of CD44 and CXCR4. ELISA was performed to measure the expression level of CXCL12 protein and CXCL12 mRNA was detected by reverse transcription‑quantitative polymerase chain reaction assays. Cell viability and apoptosis were determined with or without treatment with CXCL12 small interfering (si)RNA to examine the function of CXCL12 in Hela cells. The expression level of CD44 antigen (CD44) and C‑X‑C chemokine receptor type 4 (CXCR4) were measured using flow cytometry in the presence of CXCL12 and irradiation. In the present study, it was demonstrated that inhibition of CXCL12 reduced cell viability and increased cellular apoptosis in Hela cells treated with irradiation. Following treatment with CXCL12 siRNA, the expression level of CD44 was downregulated and the expression level of CXCR4 was upregulated. This effect of regulation additionally occurred in the presence of irradiation. In conclusion, the present data demonstrated that CXCL12 served an important role in the radioresistance of cervical cancer, suggestinh a novel therapeutic target.

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