miR‑494‑3p promotes the progression of endometrial cancer by regulating the PTEN/PI3K/AKT pathway

Zhu,Lichao; Wang,Xiaoyan; Wang,Tao; Zhu,Wenwen; Zhou,Xinge

doi:10.3892/mmr.2018.9649

miR‑494‑3p promotes the progression of endometrial cancer by regulating the PTEN/PI3K/AKT pathway

Authors:
- Lichao Zhu
- Xiaoyan Wang
- Tao Wang
- Wenwen Zhu
- Xinge Zhou
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Affiliations: Department of Geriatrics, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Rehabilitation, Zhangqiu Hospital of Traditional Chinese Medicine, Jinan, Shandong 250200, P.R. China, Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Neurosurgery, Suiping People's Hospital, Zhumadian, Henan 463100, P.R. China
Published online on: November 13, 2018 https://doi.org/10.3892/mmr.2018.9649
Pages: 581-588

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Abstract

MicroRNAs (miRs) are essential regulators in the development and progression of cancer. The role of miR-494-3p in endometrial cancer (EC) has not yet been investigated. In the present study, the expression levels of miR‑494‑3p were significantly upregulated in EC tissues compared with adjacent normal tissues. Furthermore, upregulation of miR‑494‑3p in patients with EC indicated poorer prognosis; miR‑494‑3p overexpression significantly promoted the proliferation, migration and invasion of HHUA and JEC cells in vitro. Consistently, inhibition of miR‑494‑3p in HHUA cells significantly suppressed tumor growth in vivo in a xenograft model. Additionally, phosphatase and tensin homolog (PTEN) was revealed to be a direct target of miR‑494‑3p in EC cells. Furthermore, overexpression of miR‑494‑3p inhibited PTEN expression and consequently activated the downstream phosphoinositide 3‑kinase/protein kinase B (PI3K/AKT) signialing pathway. Restoration of PTEN or inhibition of PI3K/AKT pathway also abolished miR‑494‑3p‑mediated proliferation, migration and invasion of HHUA and JEC cells. In summary, the results of the present study revealed the importance of the miR‑494‑3p/PTEN/PI3K/AKT axis in the progression of EC, which may provide novel insight into potential therapeutic targets for the treatment of EC.

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