Magnetization transfer contrast MRI in GFP‑tagged live bacteria

Righi,Valeria; Starkey,Melissa; Dai,George; Rahme,Laurence  G.; Tzika,Aria  A.

doi:10.3892/mmr.2018.9669

Magnetization transfer contrast MRI in GFP‑tagged live bacteria

Authors:
- Valeria Righi
- Melissa Starkey
- George Dai
- Laurence G. Rahme
- Aria A. Tzika
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Affiliations: NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Hospital, Harvard Medical School, Boston, MA 02114, USA, Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA 02114, USA, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA 02114, USA
Published online on: November 19, 2018 https://doi.org/10.3892/mmr.2018.9669
Pages: 617-621

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Abstract

Green fluorescent protein (GFP) is a widely utilized molecular reporter of gene expression. However, its use in in vivo imaging has been restricted to transparent tissue mainly due to the tissue penetrance limitation of optical imaging. Magnetization transfer contrast (MTC) is a magnetic resonance imaging (MRI) methodology currently utilized to detect macromolecule changes such as decrease in myelin and increase in collagen content. MTC MRI imaging was performed to detect GFP in both in vitro cells and in an in vivo mouse model to determine if MTC imaging could be used to detect infection from Pseudomonas aeruginosa in murine tissues. It was demonstrated that the approach produces values that are protein specific and concentration dependent. This method provides a valuable, non‑invasive imaging tool to study the impact of novel antibacterial therapeutics on bacterial proliferation and perhaps viability within the host system, and could potentially suggest the modulation of bacterial gene expression within the host when exposed to such compounds.

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