Expression and potential molecular mechanisms of miR‑204‑5p in breast cancer, based on bioinformatics and a meta‑analysis of 2,306 cases
- Authors:
- Published online on: December 14, 2018 https://doi.org/10.3892/mmr.2018.9764
- Pages: 1168-1184
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Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Breast cancer (BC) is the most common form of cancer among women worldwide. In the United States, ~1 in 8 women will be diagnosed with BC during their lifetime (1,2). Despite advancements in antineoplastic strategies including surgical treatment, adjuvant chemotherapy and radiotherapy, prognosis remains poor (3–6). Furthermore, the use of various prognostic markers including the cyclase associated actin cytoskeleton regulatory protein 2, lactate dehydrogenase A, AMP-activated protein kinase, Midline-2 and Claudin 12, have been reported to improve BC patient outcomes (7–10). However, in the United States, 66,120 new BC cases and 40,920 BC mortalities are likely to occur in 2018, representing growth rates of 30 and 14% relative to the previous year, respectively (11). The onset and progression of BC is a multifactorial process, associated with genetic, endocrine and external environmental factors (12). Hereditary phenomena appear in 5–10% of BC patients, and germline gene mutations, particularly in breast cancer type 1 susceptibility protein (BRCA)1 and BRCA2, closely correlate with hereditary BC (13). Further investigation is essential in understanding the complex molecular mechanisms underlying BC, while concurrently identifying more potential target genes.
miRNAs are small, endogenous non-coding RNAs, 18–22 nucleotides in length (14,15). By suppressing protein translation or enhancing the downregulation of mRNA transcripts, miRNAs have a regulatory role in the expression of target proteins (16,17). Additionally, miRNAs have been reported to have effects on the chemosensitivity, proliferation, migration, apoptosis, metastasis and invasion of BC through targeting gene regulation (18–23). The clinical features of miR-204-5p have been widely discussed in the context of various cancers, such as hepatocellular carcinoma, laryngeal squamous cell carcinoma, melanoma, oral squamous cell carcinoma, colorectal cancer, papillary thyroid carcinoma and endometrial carcinoma (24–30). Nevertheless, the expression status and molecular mechanisms underlying miR-204-5p in BC remains unclear. Therefore, in the present study, the expression of the precursor miR-204 and mature miR-204-5p in BC was investigated, using data downloaded from the Cancer Genome Atlas (TCGA), University of California Santa Cruz (UCSC) Xena and Gene Expression Omnibus (GEO) databases. In addition to the data obtained from the TCGA and UCSC Xena databases, a meta-analysis involving GEO microarrays was undertaken to evaluate the expression of miR-204 and miR-204-5p in BC. Furthermore, the putative genes selected from the intersection of the predicted genes, differentially expressed genes (DEGs) from the TCGA database, and DEGs from the GEO database were used to determine Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichments; in order to examine the molecular mechanisms underlying miR-204-5p in BC, and to construct a protein-protein interaction (PPI) network to draw interaction maps of the identified DEGs.
Materials and methods
Breast cancer samples in the TCGA and UCSC Xena databases
Precursor miR-204 expression data, as well as data on several corresponding clinical parameters, including age, gender, vital status, pathologic stage, tumor status, node status, metastasis status, estrogen receptor (ER) status, the human epidermal growth factor receptor (HER2) status and the progesterone receptor (PR) status were obtained from the TCGA database (http://cancergenome.nih.gov/) (31). Additionally, data on the expression of mature miR-204-5p were acquired from the UCSC Xena database (http://xena.ucsc.edu/).
BC microarrays in the GEO database
BC microarrays were drawn from the GEO database (https://www.ncbi.nlm.nih.gov/geo/) (32), using the following search terms: (neoplasm* OR cancer OR adenocarcinoma OR malignant* OR carcinoma OR tumor OR tumor) and (breast OR mammary). Next, BC microarrays were selected for further meta-analysis, based on the following inclusion criteria: i) Patients were diagnosed with BC tissue samples and para-carcinoma tissue samples; ii) the microRNA profile was available; and iii) corresponding clinical parameters were provided.
Predicted target genes and differentially expressed genes of miR-204-5p in breast cancer
The target genes of miR-204-5p were acquired from miRWalk 2.0 (http://zmf.umm.uni-heidelberg.de/apps/zmf/mirwalk2/) (33), which contains 12 tools: MicroT4, TargetScan, miRanda, miRNAMap, PICTAR2, RNA22, miRBridge, miRDB, PITA, miRMap, RNAhybrid, and miRWalk. To ensure accuracy, the potential predicted target genes of miR-204-5p were extracted, if they emerged more than five times among those twelve tools. Gene Expression Profiling Interactive Analysis (http://gepia.cancer-pku.cn/index.html) (34) was used to download DEGs from the TCGA database, using the following criteria: log2 |fold change (FC)|>1 and P<0.05. In addition, the Gene-Cloud of Biotechnology Information (35) was used to analyze BC microarrays obtained from the GEO database, using the following search logic: (Affymetrix) and (neoplasm* OR cancer OR adenocarcinoma OR malignant* OR carcinoma OR tumor) and (breast OR mammary). DEGs were selected from the GEO database, again under the following criteria: log2 |FC|> 1 and P<0.05.
Enrichment analyses and the protein-protein interaction network
An intersection of potential target genes, DEGs from the TCGA database and DEGs from the GEO database were plotted to obtain putative genes. Subsequently, GO (http://www.geneontology.org/) (36,37) and KEGG (https://www.genome.jp/kegg/) (38,39) analyses were undertaken to identify the potential biological processes and possible pathways of the selected putative genes in BC. A functional network graph of GO was drawn by the ORA Sample Run WEB-based Gene Set Analysis Toolkit (http://www.webgestalt.org/option.php#). Moreover, the PPI network was generated by Cytoscape 3.5.0, to build interaction maps of the putative genes (40,41). Additionally, a Spearman's correlation analysis was created to identify the correlation between miR-204-5p and the hub gene.
Statistical analysis
SPSS 22.0 (IBM Corp., Armonk, NY, USA) statistical software package was used to perform an independent-sample t-test to estimate the expression of the precursor miR-204 and mature miR-204-5p in BC tissue samples and para-carcinoma tissue samples, and to determine the expression of the precursor miR-204 in groups differentiated in terms of the aforementioned clinical parameters. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve was evaluated to assess the discriminatory capability of the precursor miR-204 for BC, in which an AUC >0.7 was considered to denote a great discriminatory capability. In addition, the Kaplan-Meier curve was undertaken to evaluate the prognostic value of the precursor miR-204 in BC, and a log-rank test was performed to compare the high and low miR-204 expression groups. STATA 12.0 (StataCorp LLC, College Station, TX, USA) was used to undertake the meta-analysis. The standard mean difference (SMD) was adopted to determine the expression of miR-204-5p in BC and para-carcinoma tissue samples. Concurrently, the heterogeneity of the BC microarrays, and the data obtained from the TCGA and UCSC Xena databases, were estimated via a heterogeneity test, with I2<50% signifying no heterogeneity. A random-effects model may be conducted if the heterogeneity existed. The sensitivity analysis was performed to seek the source of the heterogeneity. Additionally, publication bias was calculated using Deek's funnel plot dissymmetry tests, with P<0.05 indicating an obvious publication bias. Subsequently, summary (s)ROCs were determined, to calculate the discriminatory capability of miR-204-5p in BC.
Results
Expression level of the precursor miR-204 and mature miR-204-5p in breast cancer
A downregulation of miR-204 was detected in 1,077 BC tissue samples in comparison to 104 para-carcinoma tissue samples based on the TCGA database (2.284±1.983 vs. 5.775±1.391, P<0.001, Table I). The AUC of the miR-204 ROC curve was 0.9158, with a sensitivity of 87.28% and specificity of 82.69%, thus indicating that precursor miR-204 possesses a great discriminatory capability for BC (P<0.0001; Fig. 1). In addition, the expression of mature miR-204-5p was significantly decreased in 756 BC tissue samples compared with 76 para-carcinoma tissue samples based on the UCSC Xena database (1.66422±1.251283 vs. 4.55208±0.905784; P<0.001; Fig. 2A). Additionally, mature miR-204-5p also featured a great discriminatory capability for BC (P<0.0001; Fig. 2B). Additionally, downregulation of the miR-204 was determined to be significant in several groups, including individuals aged ≥60 years, those who were dead, negative PR status, positive HER2 status and pathological stage IV (all P<0.05, Table I). No significant correlation was identified between the precursor miR-204 and survival outcome in BC, as determined by the Kaplan-Meier curve (P=0.154; Fig. 3).
Meta-analysis
A total of 10 GEO microarrays containing 473 BC tissue samples and 187 para-carcinoma tissue samples were acquired (Fig. 4; Table II) (42–51). It was revealed that in two microarrays (GSE40525 and GSE44124), the expression of miR-204-5p was markedly reduced in BC tissue samples, in comparison to para-carcinoma tissue samples (both P<0.05; Fig. 5). Additionally, the ROC curve result implied that in four microarrays (GSE32922, GSE35412, GSE40525 and GSE58606), miR-204-5p possessed a great discriminatory capability for BC (all P<0.05; Fig. 6). Regarding the meta-analysis, a significant heterogeneity outcome was achieved by the heterogeneity test. Thus, a random-effects model was undertaken to calculate the SMD and 95% confidence interval, the SMD outcome demonstrated that the expression of miR-204-5p was reduced in BC tissue samples in comparison to para-carcinoma tissue samples (I2=95.7%; P<0.001; Fig. 7). The influence analysis demonstrated no significant difference (Fig. 8). Additionally, no significant publication bias was detected via Deek's funnel plot asymmetry test (P=0.14; Fig. 9). In addition, the diagnostic likelihood ratio (DLR) positive, DLR negative, diagnostic score, and odds ratio values were 3.78 (1.98–7.24), 0.27 (0.15–0.50), 2.64 (1.53–3.75) and 13.99 (4.61–42.51), respectively (Figs. 10 and 11). In addition, the prior probability and post-probability positive and negative reached 20, 49 and 6%, respectively (Fig. 12). Finally, the AUC of the sROC was 0.86 (0.82–0.89), with a sensitivity of 79% (64–88%) and a specificity of 79% (64–89%), thus indicating a great discriminatory capability of miR-204-5p for BC (Figs. 13 and 14).
Putative genes of miR-204-5p in breast cancer
In total, 1,417 and 6,158 DEGs were acquired from the TCGA and GEO databases, respectively. Additionally, 2,913 predicted target genes were obtained. The intersection was plotted and 164 putative genes were obtained for use in further bioinformatics analyses (Fig. 15).
Bioinformatics analyses
With regards to the GO analysis (Fig. 16), the putative genes of miR-204-5p were identify to have mainly participated in ‘cell development’ in biological process (BP) terms (Figs. 16A and 17), and were enriched in ‘cell surface’ in cellular component (CC) terms (Figs. 16B and 18). In addition, for molecular function (MF), the putative genes were predominantly enriched in receptor agonist activity (Figs. 16C and 19). Regarding the KEGG pathway analysis, the most enriched pathway of the putative genes was ‘endocytosis’ (Fig. 20). A total of eight pathway-associated genes were obtained: ADP-ribosylation factor 3 (ARF3), C-C chemokine receptor type 5 (CCR5), C-X-C chemokine receptor type 4 (CXCR4), receptor tyrosine-protein kinase erbB-3 (ERBB3), proteinase-activated receptor 1 (F2R), Ras-related protein Rab-10 (RAB10), Rab11 family-interacting protein 1 (RAB11FIP1) and proto-oncogene tyrosine-protein kinase receptor Ret (RET).
Furthermore, it was identified that RAB10 was significantly upregulated in BC tissue samples compared to para-carcinoma tissue samples (Fig. 21). Additionally, ROC curve analysis suggested that RAB10 possessed a great discriminatory capability for BC (Fig. 22). In terms of the PPI network, in the current study, estrogen receptor 1 (ESR1), ribonucleotide reductase regulatory subunit M2 (RRM2) and Rac GTPase activating protein 1 (RACGAP1) exhibited the highest degrees (Fig. 23). However, both ESR1 and RRM2 did not have significant negative correlations with miR-204-5p, and RRM2 mRNA expression levels were not significantly lower in para-carcinoma tissue (data not shown). Therefore, RACGAP1 was selected as the hub gene. RACGAP1 mRNA expression levels were significantly increased in BC tissue samples in comparison to para-carcinoma tissue samples (Fig. 24A). Furthermore, the ROC curve indicated that RACGAP1 had great discriminatory capability for BC (Fig. 24B). Of note, a markedly negative correlation trend was identified between RACGAP1 and miR-204-5p (P=0.0166; r=−0.3592; Fig. 24C).
Discussion
To date, many studies have reported the decreased expression of miR-204-5p in various cancers, including hepatocellular carcinoma (24), non-small cell lung cancer (52), gastric cancer (53), oral squamous cell carcinoma (27), prostate cancer (54) and esophageal cancer (55). Further, recent studies have focused on the expression of miR-204-5p in BC. For example, Wang et al (56) discovered that the expression level of miR-204-5p was obviously reduced in 24 BC tissue samples in comparison to corresponding normal tissue samples. In addition, they reported a decrease in miR-204-5p expression in two BC cell lines (MDA-MB-231 and MCF-7), compared with MCF-10A, a breast epithelial cell line (56). Shen et al (57) reported that the expression of miR-204-5p was markedly suppressed in BC cells. They also demonstrated that the expression of miR-204-5p was markedly reduced in MCF-7 cells in comparison to HBL-100 cells, which are normal breast epithelial cells. In addition, they revealed that the upregulation of miR-204-5p inhibits the invasion, proliferation and migration, and enhances the apoptosis of BC cells.
Nonetheless, in-depth research featuring abundant samples is still required. In the current study, the expression of miR-204-5p in BC was evaluated in data obtained from the TCGA, GEO, and UCSC Xena databases. In the TCGA database, a decreased trend in precursor miR-204 expression was identified in 1,077 BC tissue samples, in comparison with 104 para-carcinoma tissue samples. In addition, in the UCSC Xena database, the expression of mature miR-204-5p was notably reduced in 756 BC tissue samples, compared with 76 para-carcinoma tissue samples. Furthermore, a number of the GEO microarrays indicated that the expression of miR-204-5p was downregulated in BC tissue samples, and the SMD in the meta-analysis also showed that the expression of miR-204-5p was notably lower in 2,306 BC tissue samples, compared with the 291 para-carcinoma tissue samples. The AUCs of the ROC and sROC curves implied that t miR-204 and miR-204-5p exhibited great discriminatory capacity in BC. Next, the prognostic value of miR-204-5p in BC was determined. Prior analysis of BC samples suggested that the decreased expression of miR-204-5p correlates with poor overall survival and disease-free survival in BC (58). Ye et al (59) demonstrated that miR-204-5p had no prognostic value in BC through analyzing 563 BC tissue samples obtained from the TCGA database. In the current study, no obvious correlation was found between the precursor miR-204 and survival outcome in BC based on analyzing 1,077 BC tissue samples from TCGA database. Additionally, it was identified that miR-204 downregulation was significant in several groups, including age, vital status, PR status, HER2 status and pathological stage. Taken together with the results of these two aforementioned studies, it was hypothesized that miR-204-5p may acts as a tumor suppressor in the oncogenesis and progression of BC.
GO and KEGG analysis was performed to investigate the potential biological processes and pathways of miR-204-5p in BC. ‘Cell development’, ‘cell surface activity’ and ‘receptor agonist activity’ were considered the most enriched processes in GO analyses. Thus, it was suggested that miR-204-5p may participate these processes in BC, by targeting its corresponding target genes. However, further study is needed to verify the molecular mechanisms underlying miR-204-5p and these three processes in BC. Concurrently, in the KEGG analyses, ‘endocytosis’ was found to be the most enriched, which was associated with ARF3, CCR5, CXCR4, ERBB3, F2R, RAB10, RAB11FIP1 and RET. The expression and ROC curves of the eight pathway-related genes were estimated, and it was determined that RAB10 expression was significantly increased in BC tissue samples, compared with para-carcinoma tissue samples. In addition, RAB10 featured a great discriminatory capacity for BC within non-cancerous breast tissue samples. Hence, it was proposed that miR-204-5p may possess a vital effect on BC via genes associated with endocytosis, including RAB10. However, the role of endocytosis in BC is unclear and further investigation is urgently required.
In researching the target genes of miR-204-5p in BC, Flores-Peréz et al (60) found that transforming growth factor β receptor 2 (TGFβR2) and angiopoietin 1 (ANGPT1) are crucial in BC tumor angiogenesis; BC cell migration and proliferation decreases when TGFβR2 is suppressed, and the suppression of TGFβR2 and ANGPT1 inhibits angiogenesis. Furthermore, Zeng et al (61) identified a negative correlation trend between miR-204-5p and SIX homeobox 1 (Six1) expression in BC tissue samples, and when miR-204-5p mimics or Six1 siRNA was transfected, the expression of chromodomain helicase DNA binding protein 1 was markedly increased, thus enhancing epithelial-mesenchymal transition and affecting the invasion and migration of BC cells (61). Various target genes of miR-204-5p have been confirmed in previous studies, including traditional serrated adenoma, MX dynamin like GTPase 1, thioredoxin interacting protein, Src-associated in mitosis 68 kDa protein and forkhead box A1 (57,62–64). However, more target genes need to be determined. Accordingly, a PPI network was generated in the present study. The hub gene RACGAP1 was selected as an example for further investigation. RACGAP1 is involved in cell cytokinesis, transformation, migration, metastasis and growth (65,66). In BC specifically, it has been reported that RACGAP1 is critical in enhancing basal-like breast cancer proliferation and oncogenicity (67,68). Furthermore, in untransformed cells, RACGAP1 stimulates malignant phenotypes, and elevated RACGAP1 expression is correlated with poor BC outcomes (67,68). In the current study, RACGAP1 expression was evaluated in BC tissue sample data obtained from the TCGA database. RACGAP1 was upregulated BC tissue samples, compared with para-carcinoma tissue samples. In addition, a strong negative correlation trend was identified between RACGAP1 and miR-204-5p. Thus, it was proposed that miR-204-5p may serve a crucial role in BC by targeting RACGAP1. However, these conclusions were made based on online tools, so further in vivo and in vitro investigations should be performed to verify the molecular mechanisms of RACGAP1 and miR-204-5p in BC.
There are several limitations to the present study. First, a high degree of I2 existed in the heterogeneity test. Thus, a random effects model was conducted to reduce the degree of I2-however, it still exceeded 50%. This may be a result of using various measures and platforms used to analyze the data. The nine GEO microarrays were acquired from six countries; GSE32922, GSE44124, GSE48088 and GSE58606 were obtained from Spain, and GSE17155, GSE22981, GSE31309, GSE35412, GSE37407 and GSE40525 were obtained from Italy, the USA, Germany, Mexico, Sweden and Israel, respectively. Second, a dual luciferase reporter assay was not performed to verify the correlation between miR-204-5p and the hub gene. Thus, in-depth investigations with in vivo and in vitro experiments should be performed in the future.
In conclusion, the results of the present study identified that miR-204-5p expression was downregulated in BC tissue samples in comparison to para-carcinoma tissue samples; this suggested that miR-204-5p might function as a suppressor in the oncogenesis and advancement of BC. Furthermore, it was revealed that RACGAP1 may be a crucial target gene of miR-204-5p, and the expression of RACGAP1 was markedly increased in BC tissue samples in comparison to para-carcinoma tissue samples. Notably, a significant negative correlation was identified between RACGAP1 and miR-204-5p in BC. Therefore, it was concluded that miR-204-5p may serve a crucial role in BC by targeting RACGAP1.
Acknowledgements
Not applicable.
Funding
The ‘Future Academic Star’ Fund of the Guangxi Medical University (grant no. WLXSZX17050) supported the current study.
Availability of data and materials
The data and materials of the present study are available from the corresponding authors on reasonable request.
Authors' contributions
AGL and ZFW collected and analyzed the TCGA data. HWJ and JJZ collected and analyzed the GEO data. RQH, GC and JM collected and analyzed the UCSC data. KTC and JCZ conducted meta-analysis and wrote the manuscript. All authors read the final version of the manuscript.
Ethics approval and consent to participate
Not applicable.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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