Preparation of an acellular spinal cord scaffold to improve its biological properties

Xing,Hui; Yin,Hong; Sun,Chao; Ren,Xianjun; Tian,Yongyang; Yu,Miao; Jiang,Tao

doi:10.3892/mmr.2019.10364

Preparation of an acellular spinal cord scaffold to improve its biological properties

Authors:
- Hui Xing
- Hong Yin
- Chao Sun
- Xianjun Ren
- Yongyang Tian
- Miao Yu
- Tao Jiang
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Affiliations: Department of Orthopedics, Xinqiao Hospital, Army Medical University, Chongqing 400037, P.R. China, Emergency Department of University‑Town Hospital of Chongqing Medical University, Chongqing 401331, P.R. China
Published online on: June 6, 2019 https://doi.org/10.3892/mmr.2019.10364
Pages: 1075-1084
Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In recent years, acellular spinal cord scaffolds have been extensively studied in tissue engineering. Notably, acellular spinal cord scaffolds may be used to treat spinal cord injury; however, the method of preparation can result in low efficiency and may affect the biological properties of cells. This study aimed to use EDC crosslinking, combined with chemical extraction for tissue decellularization, in order to improve the efficiency of acellular scaffolds. To make the improved stent available for the clinical treatment of spinal cord injury, it is necessary to study its immunogenicity. Therefore, this study also focused on the adherence of rat bone marrow mesenchymal stem cells to scaffolds, and their differentiation into neuron‑like cells in the presence of suitable trophic factors. The results revealed that EDC crosslinking combined with chemical extraction methods may significantly improve the efficiency of acellular scaffolds, and may also confer better biological characteristics, including improved immunogenicity. Notably, it was able to promote adhesion of rat bone marrow mesenchymal stem cells and their differentiation into neuron‑like cells. These results suggested that the improved preparation method may be promising for the construction of multifunctional acellular scaffolds for the treatment of spinal cord injury.

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