Isatin inhibits the invasion of human neuroblastoma SH‑SY5Y cells, based on microarray analysis

Zhang,Li; Sun,Wenyan; Cao,Yi; Hou,Lin; Ju,Chuanxia; Wang,Xuefeng

doi:10.3892/mmr.2019.10378

Isatin inhibits the invasion of human neuroblastoma SH‑SY5Y cells, based on microarray analysis

Authors:
- Li Zhang
- Wenyan Sun
- Yi Cao
- Lin Hou
- Chuanxia Ju
- Xuefeng Wang
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Affiliations: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China, Experimental Center for Undergraduates of Pharmacy, School of Pharmacy, Qingdao University, Qingdao, Shandong 266021, P.R. China, Institute of Metabolic Diseases, Qingdao University, Qingdao, Shandong 266021, P.R. China
Published online on: June 12, 2019 https://doi.org/10.3892/mmr.2019.10378
Pages: 1700-1706
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neuroblastoma is the fourth most common type of extracranial malignant solid tumor in children. Isatin had been demonstrated to have inhibitory effects on neuroblastoma tumors in vivo and in vitro. The aim of the present study was to investigate the molecular mechanism related to the anti‑invasion effect of isatin on SH‑SY5Y cells using microarray analysis. The microarray data identified a number of genes to be differentially upregulated or downregulated between isatin‑treated cells and untreated controls. A large number of these genes were associated with the mTOR signaling pathway. The differentially expressed genes involved in the mTOR signaling pathway were verified further, as well as their downstream genes associated with autophagy. The results of the present study provided an insight into the potential inhibitory mechanism of isatin on neuroblastoma metastasis.

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