Open Access

Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)

  • Authors:
    • Na Lin
    • Xiu‑Juan Dong
    • Ting‑Yong Wang
    • Wen‑Ji He
    • Jing Wei
    • Hai‑Ying Wu
    • Ting‑Hua Wang
  • View Affiliations

  • Published online on: June 25, 2019     https://doi.org/10.3892/mmr.2019.10422
  • Pages: 1819-1825
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tree shrews are most closely related to the primates and so possess a number of advantages in experimental studies; they have been used as an animal model in bacterial and virus infection, cancer, endocrine system disease, and certain nervous system diseases. Their olfactory ensheathing cells (OECs) are able to release several cytokines to promote neuronal survival, regeneration and remyelination. The present study used western blot analysis to identify antibody specificity in protein extracts from whole tree shrew brains to identify the specificity of p75 nerve growth factor receptor (NGFR) derived from rabbits (75 kDa). OECs were cultured and isolated, then stained and identified using the antibodies for p75NGFR. To investigate the capacity of OECs to express cytokines and growth factors, microarray technology was used, and the analysis revealed that OECs were able to express 9,821 genes. Of these genes, 44 genes were from the neurotrophic factor family, which may indicate their potential in transplantation in vivo. The present study considered the function of OECs as revealed by other studies, and may contribute to future research.
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August-2019
Volume 20 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Lin N, Dong XJ, Wang TY, He WJ, Wei J, Wu HY and Wang TH: Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep 20: 1819-1825, 2019.
APA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., & Wang, T. (2019). Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Molecular Medicine Reports, 20, 1819-1825. https://doi.org/10.3892/mmr.2019.10422
MLA
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20.2 (2019): 1819-1825.
Chicago
Lin, N., Dong, X., Wang, T., He, W., Wei, J., Wu, H., Wang, T."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)". Molecular Medicine Reports 20, no. 2 (2019): 1819-1825. https://doi.org/10.3892/mmr.2019.10422