Open Access

Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2

  • Authors:
    • Zhuo Sun
    • Jinqi Lu
    • Muyu Wu
    • Mingyan Li
    • Lu Bai
    • Zhenduo Shi
    • Lin Hao
    • Yongping Wu
  • View Affiliations

  • Published online on: September 10, 2019     https://doi.org/10.3892/mmr.2019.10668
  • Pages: 4235-4243
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proper spindle formation and accurate chromosome segregation are essential for ensuring mitotic fidelity. Phosphatase and tensin homolog (PTEN) is a multifunctional protein, which is able to maintain the stability of the genome and chromosomes. The present study described an essential role of PTEN in regulating chromosome segregation to prevent gross genomic instability via regulation of mitotic arrest deficient 2 (MAD2). PTEN knockdown induced cell cycle arrest and abnormal chromosome segregation, which manifested as the formation of anaphase bridges, lagging chromosomes and premature chromatid separation. In addition, MAD2 was identified as a potential target of PTEN. Furthermore, the present study revealed that PTEN knockdown resulted in MAD2 degradation via the ubiquitin‑proteasomal pathway, while restoration of MAD2 expression partially ameliorated the mitotic defects induced by PTEN loss. The results from the present study proposed a novel mechanism by which PTEN maintains chromosome stability.
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November-2019
Volume 20 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun Z, Lu J, Wu M, Li M, Bai L, Shi Z, Hao L and Wu Y: Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2. Mol Med Rep 20: 4235-4243, 2019
APA
Sun, Z., Lu, J., Wu, M., Li, M., Bai, L., Shi, Z. ... Wu, Y. (2019). Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2. Molecular Medicine Reports, 20, 4235-4243. https://doi.org/10.3892/mmr.2019.10668
MLA
Sun, Z., Lu, J., Wu, M., Li, M., Bai, L., Shi, Z., Hao, L., Wu, Y."Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2". Molecular Medicine Reports 20.5 (2019): 4235-4243.
Chicago
Sun, Z., Lu, J., Wu, M., Li, M., Bai, L., Shi, Z., Hao, L., Wu, Y."Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2". Molecular Medicine Reports 20, no. 5 (2019): 4235-4243. https://doi.org/10.3892/mmr.2019.10668