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Effects of bone marrow‑derived mesenchymal stem cell transplantation on chronic obstructive pulmonary disease/obstructive sleep apnea overlap syndrome in rats
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- Published online on: September 30, 2019 https://doi.org/10.3892/mmr.2019.10714
- Pages: 4665-4673
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) are the most prevalent chronic respiratory disorders in the world (1). COPD is progressive and most commonly arises from cigarette smoking and exposure to pollutants. It is characterized by persistent respiratory symptoms and nonreversible air-flow limitation (2). Obstructive sleep apnea (OSA) is defined by repeated episodes of upper airway occlusion, which results in brief periods of breathing cessation (apnea) or a marked reduction in flow (hypopnea) during sleep (3). David Flenley (4) was the first to coin the term ‘overlap syndrome’ (OS) meaning the coexistence of COPD with OSA. Overlap syndrome has an increased mortality compared with either COPD or OSA alone. Marin et al (5) found a decreased survival rate among patients with OS compared with either COPD or OSA alone. Data are emerging that show worse deleterious cardiac changes in individuals with OS compared to those with COPD alone (6,7). Although much research still needs to be conducted, more and more researchers are vigilant about OS.
Bone marrow-derived mesenchymal stem cells (BMSCs) are pluripotent stem cells that can differentiate into osteoblasts, chondrocytes or muscle cells and even into nerve cells or hepatocytes (8). BMSCs have a good paracrine function, enabling them to secrete a variety of substances, such as neurotrophic factors, cytokines and chemokines to regulate the local microenvironment of damage, reduce inflammatory response, and promote the repair of damaged tissues. MSCs have potential therapeutic roles in chronic lung diseases (9). MSCs have the ability to regulate the immune response to tissue injury, and MSCs also promote repair in vivo and have been suggested as an attractive therapeutic candidate for various types of lung diseases, including chronic obstructive pulmonary disease (COPD), among others (10). Studies involving animal models have already demonstrated the effect of MSCs on tissue regeneration after elastase-induced emphysema, asbestos or endotoxin-induced lung injury (11,12). OSA can induce oxidative stress, inflammation and endothelial dysfunction, along with certain clinical consequences such as cardiovascular diseases and neurocognitive alterations (13–15). MSCs play an important role in the physiological response to OSA (16). MSCs also have potential therapeutic effects on COPD (17,18).
Emphysema is an important pathological characteristic of COPD, characterized by a loss of alveolar walls and permanently enlarged cavities of the terminal bronchioles (19). Oxidative stress plays an important role in the pathogenesis of COPD (20–23). The increased apoptosis in alveolar cells of the COPD model and MSCs can inhibit the apoptosis of alveolar cells (24,25). Although it has been reported that MSCs play a protective and reparative role in COPD and OSA, the data currently available on the potential role of BMSCs in overlap syndrome (OS) are still scarce. Moreover, the possible mechanisms of MSCs in OS have not been reported.
In this study, rats were used to generate the OS model by daily exposure to cigarette smoke and intermittent hypoxia. To investigate the effects and possible mechanisms of BMSCs on the OS model, BMSCs were intravenously injected into OS model rats. We found that BMSCs were able to alleviate lung injury in OS rats. Moreover, BMSCs inhibited oxidative stress and the apoptosis of endotheliocytes. The experiment validated the antioxidative stress and antiapoptotic effect of BMSCs in an OS model.
Materials and methods
Isolation and culture of BMSCs
The extraction and characterization methods of BMSCs in rats were performed as described previously (26). A total of 3 one-week-old Sprague-Dawley (SD) rats (Vital River) were sacrificed by cervical vertebra luxation. The femur and tibia of rats were removed under sterile conditions. Bone marrow cells of rats were collected from the femurs and tibias with Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare). The DMEM containing bone marrow cells was added to a Percoll gradient (Pharmacia Biotech; GE Healthcare) and centrifuged at 270 × g for 30 min. The mononuclear cells were harvested and added to DMEM containing 10% fetal bovine serum (FBS; Gibco/Thermo Fisher Scientific, Inc.). The cells were cultured at 37°C in a 5% CO2 incubator. After 24 h, nonadherent cells were removed, and the medium was replaced every 3–4 days. MSCs from the third passage were used in the subsequent experiments. BMSCs were cultured and passaged with DMEM/F12 medium (HyClone; GE Healthcare) containing 10% FBS, 100 U/ml penicillin and 0.1 mg/ml streptomycin.
Identification of molecular markers on the surface of the BMSCs
Third-generation cells were digested with trypsin (HyClone/GE Healthcare), washed with PBS l-2 times, and then centrifuged to collect the cells. A total of 1×l06 cells were taken, and 350 µl of 1X PBS was added. After mixing, flow tubes were added at 50 µl/tube. Next, 2 µl of a fluorescence-labeled monoclonal antibody CD19-Fluor 488 (cat. #NBP2-24965AF488, Novus Biologicals, USA), CD45-FITC (cat. #85-11-0461-80, eBioscience), CD29-FITC (cat. #85-11-0291-82, eBioscience), CD90-FITC (cat. #85-11-0900-85, eBioscience) or isotype control FITC (cat. #85-11-4031-81, eBioscience) was added. Next, 48 ml PBS was added to each tube and incubated at room temperature in the dark for 30 min. Cell surface markers were detected by flow cytometry (Sysmex Partec GmbH).
GFP-labeled BMSCs
Before infection, the eGFP-lentivirus (GM100202-2, Shanghai Genomeditech Co., Ltd.) was diluted in complete medium according to the manufacturer's instructions. The old medium was removed, and the virus described above and polybrene were added to the cells for infection and bleeding. After 48 h of infection, the medium containing virus was replaced with fresh medium for further culture. After 48 h, cells were injected intravenously into rats. Four weeks after the last injection, the BMSC location was observed under a confocal fluorescence microscope (Leica Microsystems GmbH).
Establishment of the OS model by cigarette smoke and intermittent hypoxia
The OS model was established by the cigarette smoke (27) and intermittent hypoxia exposure method. A total of 24 SD rats (female, 150–200 g, 6-week-old) were raised under controlled temperature (19–23°C) and humidity (40–60%), and maintained on a 12-h light/dark cycle lights. Food and water were provided ad libitum. All animal experiments were approved by the Institutional Animal Care and Use Committee of Kunming Medical University. Rats in the OS group were exposed to cigarette smoke (15 cigarettes once, 2 times daily) and intermittent hypoxia (30 times per h, 8 h per day) exposure for 8 weeks.
Grouping and BMSC transplantation
Female rats were randomly divided into three groups (n=8): Control, OS and OS+BMSCs. Rats in the control group were treated with false smoke and air exposure. From 24 h after cigarette smoke and intermittent hypoxia exposure, the rats in the OS group were injected intravenously with PBS (50 µl) and those in the OS+BMSC group were injected intravenously with 50 µl of MSCs (total number of cells: 2×106/rat) via the tail vein (1 time/week on the 7th day of each week, total 4 times). Three of the OS model rats were injected intravenously with the GFP-labeled BMSCs to investigate the location and differentiation of the transplanted BMSCs, and five of the OS model rats were injected intravenously with unlabeled BMSCs for examination with the TUNEL assay. All rats were used to perform H&E staining and immunocytochemistry assays.
Lung morphologic analysis, quantification of emphysema and lung injury score
On week 4 of the last BMSCs injection, all rats were euthanized with an overdose of anesthetic (3% sodium pentobarbital 180 mg/kg, i.p.). Lung tissues were fixed in 4% formalin, embedded in paraffin, cut into 4-µm-thick sections, and stained with hematoxylin and eosin (H&E). Histological assessment of the sections was determined using the mean alveolar number (MAN) and the mean linear intercept (MLI) method as previously described (28,29) by Image-Pro Plus 6.0 software (Media Cybernetics, Inc.). Three visual fields were observed in each slice, in which no trachea or large blood vessels were observed. The ‘+’ symbol was located in the middle of each field of each section; the number of alveolar intervals (NS) that passed the ‘+’ symbol was determined, the total length (L) of the ‘+’ symbol was measured (mm), the area of the field was determined (S), and the number of alveola (Na) in each field was counted (MLI=L/NS and MAN=Na/S). The pathological scores were detected as a reference (30). Medium size (300–1100 µm) membranous bronchi were selected to observe the following indicators: i) epithelium abscission, erosion and ulcer formation; ii) hyperplasia and hypertrophy of epithelial goblet cells; iii) mucosal epithelial ciliary lodging; iv) inflammatory cell infiltration and exudation of the airway wall; v) the formation of lymphatic nodules in the vessel wall; vi) stenosis of the small airway lumen; vii) a proliferation disorder of respiratory smooth muscle; viii) connective tissue hyperplasia of the airway wall; ix) squamous metaplasia of mucosal cells; x) wall congestion and edema; and xi) pigmentation on the wall. Each item was scored 0, 1 for mild, 2 for moderate, or 3 for severe. Three membranous bronchioles were randomly selected from the pathological sections of each rat.
Determination of SOD and MDA levels
The contents of SOD (xanthine oxidase assay method) and MDA (thiobarbituric acid assay method) were measured using respective kits purchased from Nanjing Jiancheng Bio-Engineering Institute Co., Ltd. (according to the manufacturer's instructions).
TUNEL assay
The apoptosis of alveolar septal cells was also detected using a TUNEL assay kit (cat. no. 11684817910, Roche Diagnostics). Tissue samples were fixed in 4% formaldehyde for 24 h and embedded in paraffin. Next, 4-µm-thick paraffin sections were adhered to slides. Sections were deparaffinized by heating the slides for 30 min at 60°C (or 10 min at 70°C), followed by two 5-min incubations in a xylene bath at room temperature. The tissue samples were rehydrated by transferring the slides through a graded ethanol series: 2×3 min 90% ethanol, 1×3 min 80% ethanol, 1×3 min 70% ethanol, and 1×3 min double-distilled water (ddH2O). Excess water was carefully blotted away, and 20 mg/ml proteinase K solution was pipetted to cover the sections. The mixture was then incubated for 15 min at room temperature. Following proteinase K treatment, the slides were washed 3×5 min with ddH2O. Then, the sections were covered with 50 µl of TUNEL reaction buffer for 60 min at 37°C. The slides were washed with PBS and air dried. The tissue sections were examined by confocal microscopy (magnification, ×400).
Immunofluorescence
Fixed tissues were dehydrated with various concentrations of xylene and ethanol (50% ethanol for 4 h; 75% ethanol for 4 h; 85% ethanol for 3 h; 95% ethanol for 2 h; 100% ethanol for 1 h; 100% ethanol for 1 h; 1:1 ethanol-xylene for 1 h; xylene for 1 h; and xylene for 30 min at room temperature) and embedded in paraffin. Sections (4-µm thickness) were cut from a paraffin block. Sections were dewaxed with various concentrations of xylene and ethanol (xylene for 10 min; xylene for 5 min; 100% ethanol for 5 min; 95% ethanol for 2 min; 80% ethanol for 2 min; and 70% ethanol for 2 min). Antigen retrieval was performed on the sections with 0.01 M citric acid buffer (pH 6.0) at 100°C and 80 kPa pressure. Sections were blocked by incubation with 5% v/v normal goat serum (cat. no. SL038, Beijing Solarbio Science & Technology Co., Ltd.) in PBS for 15 min at room temperature. Sections were incubated with an anti-CD34 antibody (dilution 1:100, cat. no. A10796; ABclonal Biotech Co., Ltd.) overnight at 4°C and with a CoraLite594-conjugated secondary antibody (dilution 1:100; cat. no. SA00013-4; ProteinTech Group, Inc.) for 2 h at room temperature. Nuclei were stained with 10 µg/ml DAPI in the dark for 5 min at room temperature. Sections were observed using a fluorescence microscope (magnification, ×200). Three representative fields of stained cells were analyzed using Image-Pro Plus software (version 6.0; Media Cybernetics, Inc.) to obtain the mean optical density (OD), which represents the staining strength per positive pixel.
Immunocytochemistry
Sections were incubated with an anti-CD31 antibody (dilution 1:100, A3181; ABclonal) or an anti-VWF antibody (dilution 1:100, A1335; ABclonal) overnight at 4°C followed by incubation with goat anti-rabbit IgG (H+L) horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:200, AS014, ABclonal) for 2 h at room temperature. The reactions were visualized using a 3,3′-diaminobenzidine visualization kit (Fuzhou Maixin Biotech Co., Ltd.). Sections were counterstained with hematoxylin to visualize nuclei, stained with hematoxylin for 5–10 min at room temperature, and dehydrated. Sections were examined under a light microscope at a magnification of ×200. Brown staining indicated immunoreactive positive cells, and blue staining indicated the nuclei.
Statistical analysis
All values are expressed as the mean ± SD. Comparisons were assessed with one-way analysis of variance (ANOVA) (Tukey's post hoc test) by using GraphPad Prism software version 5.0a (GraphPad, USA). All experiments were conducted at least three times. P<0.05 was considered statistically significant.
Results
Identification of BMSCs
As shown in Fig. 1A, BMSCs exhibited shapes similar to stars and spindles, with round nuclei in the center, cytoplasmic hypertrophy and a clear membrane. Flow cytometry detection showed that the third-generation BMSCs expressed high levels of CD29 (99.9%) and CD90 (99.9%), and low levels of CD19 (1.0%) and CD45 (3.1%) (Fig. 1B). These results indicate that rat BMSCs were successfully collected.
Location and differentiation of BMSCs transplanted in the lung tissue
The OS model was established by exposure to cigarette smoke and intermittent hypoxia. The model profile for each day is shown in Fig. 2A. At the beginning of the smoke exposure, the rats were agitated, irritable and pulsating, followed by less movement, eye closing, wheezing, salivating and intermittent cough. After 2~3 weeks, rats showed fur with yellowish color which easily fell off, a dispirited demeanor, a decline in appetite, an emaciated bodily form, and a decrease in activity ability. To evaluate the effect of BMSCs, we used an OS model with intravenous injection of BMSCs (Fig. 2B). GFP-labeled BMSCs showed green fluorescence, and green fluorescent cells (BMSCs) were observed in the alveolar walls of lung tissues in the OS+BMSC group (Fig. 2C). Immunofluorescence detection found that some cells had both green and red fluorescent (CD34-positive) cells (Fig. 2C). There are three important cell types in alveolar tissues: Alveolar type I epithelial cells, alveolar type II epithelial cells and endotheliocytes. These three types of cells are important for maintaining the integrity of alveolar tissue, and the loss of one of these cell types directly affects the integrity of the alveolar tissue. CD34 is a marker of endotheliocytes; thus, we believe that some of the transplanted BMSCs had differentiated into endotheliocytes of the alveolar walls. Our results suggest that BMSCs can migrate into alveolar walls and differentiate into the endotheliocytes of alveolar walls.
BMSC transplantation reduces emphysematous changes in the OS rat model
H&E staining was performed to detect emphysema and pathological scores in the lung tissues of rats. We quantified the enlargement of the alveolar spaces and alveolar septum fracture by MLI, MAN and the pathological scores in the lung tissue by the lung injury score. The alveolar structure was intact in the control group (Fig. 3A and D). In the OS group, there were emphysematous changes, the alveolar size was different, the alveolar number was decreased, the alveolar space was enlarged, and the alveolar septum was fractured (Fig. 3B). Moreover, in the OS group, a small number of bronchial epithelial cells were shed, and the bronchial epithelial cells exhibited edema and degeneration; the bronchial tubes were irregular in shape and narrow in the lumen; the alveolar walls were thickened; the alveoli fused into a large cystic cavity; and inflammatory cell infiltration in bronchial and lung tissue was observed in the OS group (Fig. 3E). In the OS+BMSC group, the inflammatory cell infiltration and emphysematous changes were significantly reduced after BMSC transplantation compared with before BMSC transplantation (Fig. 3C and F).
The MLI and pathological scores were significantly increased, and the MAN was significantly decreased in the OS group compared with the control group. Compared with the OS group, the MLI and pathological scores were significantly reduced and the MAN was promoted in the OS+BMSC group (Fig. 3G-I). BMSC transplantation alleviated lung injury in the OS rats.
BMSC transplantation has an antioxidant effect on OS rats
We also investigated the functions of BMSCs on oxidative stress in OS rats. As shown in Table I, there was an increase in the MDA content and a decrease in SOD activity in serum and lung tissues in the OS group, while BMSCs attenuated the elevated MDA content and activation of SOD.
BMSC transplantation reduces endotheliocyte apoptosis in the alveolar walls of OS rats
The apoptotic cells under fluorescence microscopy displayed green fluorescence, while the endotheliocytes under fluorescence microscopy fluoresced red. The co-expression of red and green fluorescence indicated apoptotic endotheliocytes. As shown in Fig. 4A, few apoptotic cells were observed in the alveolar wall of the control group, and apoptosis was more obvious in the OS group than it was in the control group. BMSC transplantation significantly reduced endotheliocyte apoptosis in the alveolar walls of OS rats. Quantitative analysis showed that the apoptosis index of endotheliocytes in the OS+BMSC group was significantly lower than that in the OS group (Fig. 4B). These results suggest that BMSCs can reduce endotheliocyte apoptosis in the alveolar walls of OS rats.
BMSC transplantation promotes the expression of CD31 and VWF in the alveolar walls of OS rats
CD31 and VWF are markers of endotheliocytes. The levels of CD31 and VWF were detected by an immunohistochemical assay. As shown in Fig. 5A and C, the cytoplasm of alveolar wall cells in the lung tissue sections of the transplanted rats was stained brown. A small amount of brown was observed in the alveolar wall of the OS group compared with the control group. The quantitative results (Fig. 5B and D) of the immunohistochemical assay are consistent with Fig. 5A and C. BMSC transplantation significantly increased the brown staining in the alveolar walls of OS rats. These results indicate that BMSCs suppressed the apoptosis of endotheliocytes in the alveolar walls of OS rats.
Discussion
Several studies have confirmed that individuals with chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) have more profound nocturnal oxygen desaturation and sleep disturbances compared with those with either disease alone (31). Marin et al (5) found decreased survival among patients with overlap syndrome (OS) compared to those with either COPD or OSA alone. Bone marrow-derived mesenchymal stem cells (BMSCs) are stem cells that are found in connective tissues throughout the whole body; they are easy to harvest, easy to culture, quick to proliferate and weakly immunogenic (32–34). Previous studies have shown that BMSCs can migrate into damaged lung tissue (35,36). Moreover, BMSCs not only differentiate into lung epithelial cells in damaged lung tissue (37) but also secrete various growth factors and cytokines in response to lung injury (38). Some studies have focused on the effect of BMSCs on COPD alone or OSA alone, but few studies have focused on the OS model.
One of the characteristics of OSA is intermittent hypoxemia, and smoking is the main cause of COPD development. Thus, we constructed the OS rat model with exposure to cigarette smoke and intermittent hypoxia, yet the intermittent hypoxia model cannot simulate upper airway obstruction. At present, there are no relevant studies on spontaneous and natural OS animal models, and there is no accepted evaluation standard for the OS model. Theoretically, the establishment of the model can be judged based on the evaluation criteria of OSA and COPD animal models, and the main pathological characteristics of the two can determine the success of the model. Building an ideal OS animal model requires more exploration and practice. Compared with the control group, the OS rats were in poor condition, with acidosis, hypoxemia and carbon dioxide retention, and right ventricular hypertrophy (data not shown). In this study, we first examined the effects of BMSC transplantation in the OS model induced by cigarette smoke and intermittent hypoxia and its potential mechanisms in vitro. Our results revealed that BMSC transplantation relieved emphysema and lung injury in the OS model by inhibiting oxidative stress and apoptosis of endotheliocytes in the alveolar walls. We created an OS model induced by cigarette smoke and intermittent hypoxia. Alveolar wall rupture and emphysematous changes were observed in the OS group. The MLI and pathological scores were higher and the MAN was lower in the OS group than these parameters in the control group. Moreover, the malondialdehyde (MDA) content was increased and the superoxide dismutase (SOD) activity was decreased in the OS group compared with the control group. These results suggest that cigarette smoke and intermittent hypoxia could promote oxidative stress, emphysema, alveolar congestion, alveolar wall edema and inflammatory cell infiltration and increase the lung injury score. The BMSC transplantation group exhibited significantly alleviated lung injury compared with the OS group. SOD is an important free radical scavenger in the body that can protect cell membranes from oxidation and lipid peroxidation (39). Decreased SOD activity in blood and lung tissues is one of the manifestations of smoking-induced COPD damage. MDA is a product of lipid peroxidation induced by free radical attack on biofilms. The MDA level can reflect the degree of lipid peroxidation in the body (40), and its level in serum and lung can indirectly reflect the degree of cell damage. The increased SOD activity and decreased MDA content in the serum and lung of the OS+BMSC group rats, suggest that BMSCs can enhance the activity of free radical scavenging enzymes and enhance the antioxidant capacity and reduce the damage caused by lipid peroxidation.
To investigate whether the transplanted BMSCs could migrate into the lung in OS rats, we marked BMSCs with an eGFP lentivirus before injection. We also detected endotheliocytes by CD34 immunofluorescence. We observed green fluorescence in the alveolar walls and its co-expression with CD34. This implied that BMSCs can migrate to the injury lung tissues, but not only lung tissues. We found that BMSCs also can migrate into the aorta (data not shown). Moreover, decreased apoptosis of endotheliocytes in alveolar walls was observed in the OS+BMSC group, and a high rate of apoptosis was found in the OS group. These results suggest that BMSCs can migrate into lung tissues and differentiate into endotheliocytes in alveolar walls, and BMSC transplantation markedly reduced the apoptosis of endotheliocytes in alveolar walls, which may represent one mechanism for emphysema therapy. Some studies have indicated that the apoptosis of alveolar type II epithelial cells is directly associated with emphysema (41,42) and that MSCs can differentiate into alveolar type I and II epithelial cells (43–45). These studies indicate that MSCs can differentiate into alveolar cells. In the present study, transplanted BMSCs with CD34-positive expression differentiated into alveolar wall endotheliocytes, and the apoptosis of endotheliocytes in the alveolar walls is important for OS, which may help to alleviate emphysema. We also examined the expression of the endothelial cell markers CD31 and VWF by immunohistochemical assay. The results suggested that BMSCs suppress the apoptosis of endothelial cells, which agrees with previous TUNEL results. There was a possibility that the release of growth factors and structural support may be a determinant for the regenerative effects observed following treatment with BMSCs. Since BMSCs have strong proliferative properties, they are theoretically tumorigenic in tissue or cell transplantation (46). Therefore, the numbers of BMSCs for treatment should be strictly controlled, so that the excessive accumulation of BMSCs do not cause negative effects. BMSCs can migrate to damaged tissues, so they are present not only in the lungs, but also in other tissues, such as the aorta and the heart. This indicates that the OS model can lead not only to lung injury but also to other organ injury. We will study the repair effect of BMSCs on other organs in OS rats in further research. There is also the question of whether the presence of undifferentiated BMSCs causes damage to organs, since excessive injection increases the risk of tumors. Therefore, the use of BMSCs must be strictly controlled within a safe range, which requires more in-depth research.
In summary, we demonstrated the therapeutic effect of BMSC administration in OS rats induced by cigarette smoke and intermittent hypoxia. BMSCs can suppress pulmonary emphysema and oxidative stress in OS rats. Moreover, BMSCs were able to migrate into the alveolar walls and differentiate into endotheliocytes in the alveolar wall, inhibiting endotheliocyte apoptosis. This experiment confirms that the inhibition of the progression of emphysema by BMSCs in the OS model may be through the differentiation of BMSCs into endotheliocytes consequently suppressing endotheliocyte apoptosis and by their antioxidative stress function. However, further elucidation of the relationship between BMSCs and endotheliocyte proliferation and whether they can promote endotheliocyte proliferation remain to be further studied. Our study indicates that BMSCs are potent and may serve as a basis for clinical trials with OS patients in the near future.
Acknowledgements
Not applicable.
Funding
This research was supported by the National Natural Science Foundation of China (no. 81560010) and the Science and Technology Planning Project of Yunnan Province [no. 2017FE467(−100)].
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
MC, ZH and ZJ conceived and designed the study. HB, XP, JH, LH and XH performed the experiments. JD, KZ, LW, QW and XG collected and processed the data. MC, ZH and QW wrote the manuscript. MC, ZH, XG and ZJ reviewed and edited the manuscript. All authors read and approved the manuscript and agree to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ethics approval and consent to participate
The animal experiments were approved by the Animals Ethics Committee of Kunming Medical University and the Research Medical Ethics Committee of Kunming Medical University.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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