Influence of reaming intramedullary nailing on MSC population after surgical treatment of patients with long bone fracture

Sperelakis,Ioannis; Tsitoura,Eliza; Koutoulaki,Chara; Mastrodimou,Semeli; Tosounidis,Theodoros   H.; Spandidos,Demetrios   A.; Antoniou,Katerina   M.; Kontakis,George

doi:10.3892/mmr.2020.11320

Influence of reaming intramedullary nailing on MSC population after surgical treatment of patients with long bone fracture

Authors:
- Ioannis Sperelakis
- Eliza Tsitoura
- Chara Koutoulaki
- Semeli Mastrodimou
- Theodoros H. Tosounidis
- Demetrios A. Spandidos
- Katerina M. Antoniou
- George Kontakis
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Affiliations: Department of Orthopedics and Traumatology, University of Crete School of Medicine, 71003 Heraklion, Greece, Department of Respiratory Medicine, University General Hospital of Heraklion, Laboratory of Molecular and Cellular Pneumonology, Medical School, University of Crete, 71003 Heraklion, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece
Published online on: July 9, 2020 https://doi.org/10.3892/mmr.2020.11320
Pages: 2521-2527
Copyright: © Sperelakis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Reamed intramedullary nailing (RIN) is a surgical method of choice for treatment of diaphyseal fractures. This procedure affects the biological environment of bone tissue locally and systemically. This study investigated the influence of RIN on mesenchymal stem cells (MSCs) in patients with long bone fractures. The axis of C-X-C motif chemokine receptor 4 (CXCR4)/stromal cell-derived factor 1 (SDF‑1) was selected since it is considered as major pathway for MSC homing and migration. Iliac crest bone marrow (IC‑BM) samples and blood samples were collected at two different time points. One sample was collected before the RIN (BN) and the other immediately after RIN (AN). BM‑MSCs were cultured and RT‑qPCR was performed for CXCR4 mRNA levels and ELISA for the SDF‑1 sera levels. The experimental study revealed that there was a correlation between the increase of SDF‑1 levels in peripheral blood and a decrease in the levels of CXCR4 in MSCs in the IC‑BM following RIN. The levels of SDF‑1 showed a significant increase in the sera of patients after RIN. In conclusion, the present study is the first providing evidence of the effects of RIN on MSC population via the CXCR4/SDF‑1 axis. The levels of serum SDF‑1 factor were elevated after RIN while increased levels of SDF‑1 in peripheral blood were inversely correlated with the mRNA levels of CXCR4 on BM‑MSCs after RIN. Therefore, this study contributes to enlighten the systematic effects of RIN on the population of MSCs at a cellular level.

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