Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Lv,Dongjin; Bi,Qing; Li,Yunxia; Deng,Jie; Wu,Na; Hao,Shu; Zhao,Mingli

doi:10.3892/mmr.2021.11830

Long non‑coding RNA MEG3 inhibits cell migration and invasion of non‑small cell lung cancer cells by regulating the miR‑21‑5p/PTEN axis

Authors:
- Dongjin Lv
- Qing Bi
- Yunxia Li
- Jie Deng
- Na Wu
- Shu Hao
- Mingli Zhao
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Affiliations: Department of Medical Oncology, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan 650118, P.R. China, Department of Pharmacy, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan 650118, P.R. China
Published online on: January 6, 2021 https://doi.org/10.3892/mmr.2021.11830
Article Number: 191
Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) are involved in the occurrence and progression of numerous types of cancer. The aim of the present study was to evaluate the effect of the lncRNA maternally expressed gene 3 (MEG3) on the migration and invasion of non‑small cell lung cancer (NSCLC) H1299 and PC9 cells. Reverse transcription‑quantitative (RT‑q)PCR analysis showed that MEG3 was downregulated in NSCLC PC9 and H1299 cells. Additionally, bioinformatics analysis indicated that MEG3 sponges microRNA (miR)‑21‑5p; miR‑21‑5p was predicted to target the phosphatase and tensin homolog (PTEN) 3'‑untranslated region sequence. MEG3 overexpression led to miR‑21‑5p suppression and PTEN upregulation in PC9 and H1299 cells, as detected by RT‑qPCR. Subsequently, western blot analysis confirmed that MEG3 overexpression enhanced PTEN expression levels and inhibited the PI3K/AKT signaling pathway in NSCLC cells. These effects were attenuated by miR‑21‑5p. Dual luciferase assay supported the sponging effect of MEG3 on miR‑21‑5p and validated the direct interaction between miR‑21‑5p and PTEN. Furthermore, Transwell assay demonstrated that MEG3 overexpression had an inhibitory effect on cell migration and invasion. MEG3 overexpression also mediated epithelial‑to‑mesenchymal transition by significantly enhancing E‑cadherin and decreasing N‑cadherin, Vimentin and matrix metalloprotein 9 expression levels in NSCLC cells, as indicated by western blot analysis. These changes were partially reversed by an miR‑21‑5p mimic. These results indicated that MEG3 acted as a tumor suppressor that inhibited NSCLC cell migration and invasion via sponging miR‑21‑5p, which, in turn, enhanced the expression levels of PTEN, in part via the PI3K/AKT signaling pathway. The results of the present study have suggested the potential of MEG3 as a novel therapeutic target for NSCLC treatment.

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