Open Access

Paeonol alleviates lipopolysaccharide‑induced hepatocytes injury through alteration of mitochondrial function and NF‑κB translocation

  • Authors:
    • Shouzhu Xu
    • Jie Xu
    • Ting Hao
    • Yu Yan
    • Shihao Zhang
    • Aihong Li
    • Chuandao Shi
    • Qiling Liu
    • Jing Zhao
  • View Affiliations

  • Published online on: September 7, 2021     https://doi.org/10.3892/mmr.2021.12419
  • Article Number: 779
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Sepsis is a severe disease, with high mortality. Permanent organ damage caused by sepsis reduces the quality of life of surviving patients. The liver is an easily damaged organ in sepsis and sepsis‑associated liver injury foretells a poor prognosis. Unfortunately, there are no effective treatments or drugs to solve this problem. Therefore, strategies or novel drugs are urgently required to protect against liver dysfunction in sepsis. In the present study, lipopolysaccharide (LPS) was used to establish a model of liver injury in vitro. The data demonstrated that pretreatment of L02 human normal hepatocytes with paeonol (PAE) alleviated LPS‑induced cell injury and decreased the levels of alanine aminotransferase and aspartate transaminase, indicating a protective effect of PAE. Further experiments demonstrated that PAE increased LPS‑decreased L02 cell viability, the levels of superoxide dismutase and Bcl‑2 expression. PAE decreased LPS‑increased cell apoptosis, intracellular reactive oxygen species and the expression levels of Bax and cleaved‑caspase‑3. PAE decreased LPS‑promoted mitochondrial depolarization and nuclear translocation of NF‑κB. In conclusion, PAE alleviated LPS‑induced liver injury via alteration of mitochondrial function and NF‑κB translocation. Therefore, PAE has potential for the treatment of sepsis.
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November-2021
Volume 24 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu S, Xu J, Hao T, Yan Y, Zhang S, Li A, Shi C, Liu Q and Zhao J: Paeonol alleviates lipopolysaccharide‑induced hepatocytes injury through alteration of mitochondrial function and NF‑κB translocation. Mol Med Rep 24: 779, 2021.
APA
Xu, S., Xu, J., Hao, T., Yan, Y., Zhang, S., Li, A. ... Zhao, J. (2021). Paeonol alleviates lipopolysaccharide‑induced hepatocytes injury through alteration of mitochondrial function and NF‑κB translocation. Molecular Medicine Reports, 24, 779. https://doi.org/10.3892/mmr.2021.12419
MLA
Xu, S., Xu, J., Hao, T., Yan, Y., Zhang, S., Li, A., Shi, C., Liu, Q., Zhao, J."Paeonol alleviates lipopolysaccharide‑induced hepatocytes injury through alteration of mitochondrial function and NF‑κB translocation". Molecular Medicine Reports 24.5 (2021): 779.
Chicago
Xu, S., Xu, J., Hao, T., Yan, Y., Zhang, S., Li, A., Shi, C., Liu, Q., Zhao, J."Paeonol alleviates lipopolysaccharide‑induced hepatocytes injury through alteration of mitochondrial function and NF‑κB translocation". Molecular Medicine Reports 24, no. 5 (2021): 779. https://doi.org/10.3892/mmr.2021.12419