Open Access

Intermittent hypoxia mimicking obstructive sleep apnea aggravates early brain injury following ICH via neuroinflammation and apoptosis

  • Authors:
    • Wenjing Fei
    • Wei Jiao
    • Xiaoyan Feng
    • Xufeng Chen
    • Yuhai Wang
  • View Affiliations

  • Published online on: September 24, 2021     https://doi.org/10.3892/mmr.2021.12464
  • Article Number: 824
  • Copyright: © Fei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Spontaneous intracerebral hemorrhage (ICH) is a subtype of stroke associated with high mortality and morbidity due to the lack of effective therapy. Obstructive sleep apnea (OSA) has been reported to aggravate early brain injury (EBI) and worsen the overall outcome of patients with ICH. However, the precise role of OSA‑mediated neuroinflammation and apoptosis following ICH has not been confirmed. The present study aimed to investigate the neuronal damage induced by OSA and the potential molecular mechanisms by which ICH‑induced EBI regulates neural apoptosis in a C57BL/6 mouse ICH model. Mortality, neurological score, brain water content and neuronal death were evaluated by Evans blue extravasation, TUNEL staining, ELISA, analysis of reactive oxygen species/lipid peroxidation and western blotting. The results showed that OSA induction decreased survival rate, neurological score and neuron survival and upregulated the protein expression levels of Caspase‑3, Bax, cytokines IL‑1β, IL‑6 and TNF‑α and NF‑κB, which indicated that OSA‑mediated induction of apoptosis and neuroinflammation aggravated neuronal death following ICH. The molecular mechanism was partly dependent on the activating transcription factor/CHOP pathway. Taken together, the results demonstrated that OSA worsens neurological outcomes in mice and increases neuronal death by enhancing neural apoptosis and neuroinflammation.
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November-2021
Volume 24 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Fei W, Jiao W, Feng X, Chen X and Wang Y: Intermittent hypoxia mimicking obstructive sleep apnea aggravates early brain injury following ICH via neuroinflammation and apoptosis. Mol Med Rep 24: 824, 2021.
APA
Fei, W., Jiao, W., Feng, X., Chen, X., & Wang, Y. (2021). Intermittent hypoxia mimicking obstructive sleep apnea aggravates early brain injury following ICH via neuroinflammation and apoptosis. Molecular Medicine Reports, 24, 824. https://doi.org/10.3892/mmr.2021.12464
MLA
Fei, W., Jiao, W., Feng, X., Chen, X., Wang, Y."Intermittent hypoxia mimicking obstructive sleep apnea aggravates early brain injury following ICH via neuroinflammation and apoptosis". Molecular Medicine Reports 24.5 (2021): 824.
Chicago
Fei, W., Jiao, W., Feng, X., Chen, X., Wang, Y."Intermittent hypoxia mimicking obstructive sleep apnea aggravates early brain injury following ICH via neuroinflammation and apoptosis". Molecular Medicine Reports 24, no. 5 (2021): 824. https://doi.org/10.3892/mmr.2021.12464