Open Access

Proteomics changes after negative pressure wound therapy in diabetic foot ulcers

  • Authors:
    • Zeguo Jia
    • Lei Liu
    • Shiqi Zhang
    • Xiaotong Zhao
    • Li Luo
    • Yizhong Tang
    • Bing Shen
    • Mingwei Chen
  • View Affiliations

  • Published online on: October 1, 2021     https://doi.org/10.3892/mmr.2021.12474
  • Article Number: 834
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Label‑free quantitative mass spectrometry was used to analyze the differences in the granulation tissue protein expression profiles of patients with diabetic foot ulcers (DFUs) before and after negative‑pressure wound therapy (NPWT) to understand how NPWT promotes the healing of diabetic foot wounds. A total of three patients with DFUs hospitalized for Wagner grade 3 were enrolled. The patients received NPWT for one week. The granulation tissue samples of the patients prior to and following NPWT for one week were collected. The protein expression profiles were analyzed with label‑free quantitative mass spectrometry and the differentially expressed proteins (DEPs) in the DFU patients prior to and following NPWT for one week were identified. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to annotate the DEPs and DEP‑associated signaling pathways. Western blotting and ELISA were performed to validate the results. By comparing the differences in the protein profiles of granulation tissue samples prior to and following NPWT for one week, 36 proteins with significant differences were identified (P<0.05); 33 of these proteins were upregulated and three proteins were downregulated. NPWT altered proteins mainly associated with antioxidation and detoxification, the cytoskeleton, regulation of the inflammatory response, complement and coagulation cascades and lipid metabolism. The functional validation of the DEPs demonstrated that the levels of cathepsin S in peripheral blood and granulation tissue were significantly lower than those prior to NPWT (P<0.05), while the levels of protein S isoform 1, inter α‑trypsin inhibitor heavy chain H4 and peroxiredoxin‑2 in peripheral blood and granulation tissue were significantly higher than those prior to NPWT (P<0.05). The present study identified multiple novel proteins altered by NPWT and laid a foundation for further studies investigating the mechanism of action of NPWT.
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December-2021
Volume 24 Issue 6

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Copy and paste a formatted citation
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Spandidos Publications style
Jia Z, Liu L, Zhang S, Zhao X, Luo L, Tang Y, Shen B and Chen M: Proteomics changes after negative pressure wound therapy in diabetic foot ulcers. Mol Med Rep 24: 834, 2021.
APA
Jia, Z., Liu, L., Zhang, S., Zhao, X., Luo, L., Tang, Y. ... Chen, M. (2021). Proteomics changes after negative pressure wound therapy in diabetic foot ulcers. Molecular Medicine Reports, 24, 834. https://doi.org/10.3892/mmr.2021.12474
MLA
Jia, Z., Liu, L., Zhang, S., Zhao, X., Luo, L., Tang, Y., Shen, B., Chen, M."Proteomics changes after negative pressure wound therapy in diabetic foot ulcers". Molecular Medicine Reports 24.6 (2021): 834.
Chicago
Jia, Z., Liu, L., Zhang, S., Zhao, X., Luo, L., Tang, Y., Shen, B., Chen, M."Proteomics changes after negative pressure wound therapy in diabetic foot ulcers". Molecular Medicine Reports 24, no. 6 (2021): 834. https://doi.org/10.3892/mmr.2021.12474