Salivary microbiota analysis of patients with membranous nephropathy

Luan,Shaodong; Zhang,Shuyuan; Pan,Litao; Hu,Wenjun; Cui,Haihong; Wei,Xing; Lin,Renyong; Li,Chundi; Zeng,Ping; Wang,Xi; Li,Weilong; Xu,Zigan; Zhang,Yingwei; Hu,Bo; Gao,Hanchao

doi:10.3892/mmr.2022.12706

Salivary microbiota analysis of patients with membranous nephropathy

Authors:
- Shaodong Luan
- Shuyuan Zhang
- Litao Pan
- Wenjun Hu
- Haihong Cui
- Xing Wei
- Renyong Lin
- Chundi Li
- Ping Zeng
- Xi Wang
- Weilong Li
- Zigan Xu
- Yingwei Zhang
- Bo Hu
- Hanchao Gao
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Affiliations: Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong 518110, P.R. China, Department of Acupuncture and Massage, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518037, P.R. China, Department of Anesthesiology, The 305 Hospital of PLA, Beijing 100036, P.R. China, Department of Nephrology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong 518110, P.R. China
Published online on: March 31, 2022 https://doi.org/10.3892/mmr.2022.12706
Article Number: 190
Copyright: © Luan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The oral microbiota are closely related to human health. Nonetheless, to the best of our knowledge, their relationship with membranous nephropathy (MN) remains unstudied. The saliva microbiota collected from 22 patients with MN and 15 healthy controls were analyzed by next‑generation sequencing, and bioinformatics analysis of the 16S ribosomal RNA gene was subsequently carried out. The Chao1 and Shannon indices in patients with MN were higher than those in healthy controls. Analysis of similarities revealed that the oral microbiota in the patient group were significantly different from those in the healthy controls. At the genus level, the abundance of Alloprevotella, Granulicatella, Prevotella, Streptococcus and Prevotella_7 was markedly higher in patients with MN than in healthy controls. Six operational taxonomic units (OTUs; OTU5, OTU28, OTU9, OTU15, OTU33 and OTU38) were found to be markedly correlated with the clinical factors creatinine, proteinuria in 24 h, estimated glomerular filtration rate and systolic blood pressure. A total of 28 Kyoto Encyclopedia of Genes and Genomes pathways were obtained from the significant OTUs. The oral microbiota of patients with MN were investigated and it was found that OTU5, OTU28, OTU9, OTU15, OTU33 and OTU38 may be used as biomarkers. The present findings may assist in the diagnosis of patients with MN.

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