Open Access

Apolipoprotein E mimetic peptide COG1410 alleviates blood‑brain barrier injury in a rat model of ischemic stroke

  • Authors:
    • Yunwen Xue
    • Minhua Gu
    • Cuilan Chen
    • Yujian Yao
    • Yuzhen Li
    • Guohu Weng
    • Yong Gu
  • View Affiliations

  • Published online on: March 3, 2023     https://doi.org/10.3892/mmr.2023.12972
  • Article Number: 85
  • Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Blood‑brain barrier (BBB) damage is one of the main causes of poor outcomes and increased mortality rates following cerebral ischemia‑reperfusion injury. Apolipoprotein E (ApoE) and its mimetic peptide have been previously reported to exhibit potent neuroprotective properties in various central nervous system disease models. Therefore, the present study aimed to investigate the possible role of the ApoE mimetic peptide COG1410 in cerebral ischemia‑reperfusion injury and its potential underlying mechanism. Male SD rats were subjected to 2 h middle cerebral artery occlusion followed by 22 h reperfusion. Evans blue leakage and IgG extravasation assays results revealed that COG1410 treatment significantly reduced BBB permeability. In addition, in situ zymography and western blotting were used to prove that COG1410 was able to downregulate the activities of MMPs and upregulate the expression of occludin in the ischemic brain tissue samples. Subsequently, COG1410 was found to significantly reverse microglia activation while also suppressing inflammatory cytokine production, according to immunofluorescence signal of Iba‑1 and CD68 and protein expression of COX‑2. Consequently, this neuroprotective mechanism mediated by COG1410 was further tested using the BV2 cell line in vitro, which was exposed to oxygen glucose deprivation followed by reoxygenation. The mechanism of COG1410 was found to be mediated, as least partly, through the activation of triggering receptor expressed on myeloid cells 2. In conclusion, the data suggest that COG1410 can alleviate BBB injury and neuroinflammation following ischemic stroke.
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April-2023
Volume 27 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xue Y, Gu M, Chen C, Yao Y, Li Y, Weng G and Gu Y: Apolipoprotein E mimetic peptide COG1410 alleviates blood‑brain barrier injury in a rat model of ischemic stroke. Mol Med Rep 27: 85, 2023
APA
Xue, Y., Gu, M., Chen, C., Yao, Y., Li, Y., Weng, G., & Gu, Y. (2023). Apolipoprotein E mimetic peptide COG1410 alleviates blood‑brain barrier injury in a rat model of ischemic stroke. Molecular Medicine Reports, 27, 85. https://doi.org/10.3892/mmr.2023.12972
MLA
Xue, Y., Gu, M., Chen, C., Yao, Y., Li, Y., Weng, G., Gu, Y."Apolipoprotein E mimetic peptide COG1410 alleviates blood‑brain barrier injury in a rat model of ischemic stroke". Molecular Medicine Reports 27.4 (2023): 85.
Chicago
Xue, Y., Gu, M., Chen, C., Yao, Y., Li, Y., Weng, G., Gu, Y."Apolipoprotein E mimetic peptide COG1410 alleviates blood‑brain barrier injury in a rat model of ischemic stroke". Molecular Medicine Reports 27, no. 4 (2023): 85. https://doi.org/10.3892/mmr.2023.12972