Open Access

VAV3 regulates glioblastoma cell proliferation, migration, invasion and cancer stem‑like cell self‑renewal

  • Authors:
    • Rui Miao
    • Dong Huang
    • Kaitao Zhao
    • Yang Li
    • Xiaomei Zhang
    • Yi Cheng
    • Na Guo
  • View Affiliations

  • Published online on: March 22, 2023     https://doi.org/10.3892/mmr.2023.12981
  • Article Number: 94
  • Copyright: © Miao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma multiforme (GBM; World Health Organization grade IV) is one of the most common and aggressive malignant brain tumors and has no effective treatment. Therefore, elucidation of the molecular mechanism of glioma development is very important for finding new therapeutic strategies. The present study evaluated the expression level of Vav guanine nucleotide exchange factor 3 (VAV3) using bioinformatics analysis and demonstrated that VAV3 was overexpressed in human glioblastoma and associated with patient survival. Knock down of VAV3 using shRNA in glioblastoma cells significantly inhibited glioblastoma cell migration, invasion and proliferation. Furthermore, downregulation of VAV3 expression inhibited the stem cell self‑renewal capacity and decreased the expression levels of the stem cell markers Nestin and Sox2. Bioinformatic analysis demonstrated that VAV3 was a target gene of miR‑218. Furthermore, overexpression of VAV3 markedly reversed the tumor suppressor effect of miR‑218 in glioblastoma cell. These findings suggested that VAV3 could be a potential biomarker and therapeutic target for glioblastoma.
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April-2023
Volume 27 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Miao R, Huang D, Zhao K, Li Y, Zhang X, Cheng Y and Guo N: VAV3 regulates glioblastoma cell proliferation, migration, invasion and cancer stem‑like cell self‑renewal. Mol Med Rep 27: 94, 2023.
APA
Miao, R., Huang, D., Zhao, K., Li, Y., Zhang, X., Cheng, Y., & Guo, N. (2023). VAV3 regulates glioblastoma cell proliferation, migration, invasion and cancer stem‑like cell self‑renewal. Molecular Medicine Reports, 27, 94. https://doi.org/10.3892/mmr.2023.12981
MLA
Miao, R., Huang, D., Zhao, K., Li, Y., Zhang, X., Cheng, Y., Guo, N."VAV3 regulates glioblastoma cell proliferation, migration, invasion and cancer stem‑like cell self‑renewal". Molecular Medicine Reports 27.4 (2023): 94.
Chicago
Miao, R., Huang, D., Zhao, K., Li, Y., Zhang, X., Cheng, Y., Guo, N."VAV3 regulates glioblastoma cell proliferation, migration, invasion and cancer stem‑like cell self‑renewal". Molecular Medicine Reports 27, no. 4 (2023): 94. https://doi.org/10.3892/mmr.2023.12981