Open Access

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)

  • Authors:
    • Qingqing Liu
    • Xiaojuan Jin
    • Jun Cheng
    • Huajun Zhou
    • Yingjie Zhang
    • Yuzhu Dai
  • View Affiliations

  • Published online on: April 3, 2023     https://doi.org/10.3892/mmr.2023.12991
  • Article Number: 104
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Infectious diseases are a major global cause of morbidity and mortality, seriously affecting public health and socioeconomic stability. Since infectious diseases can be caused by a wide variety of pathogens with similar clinical manifestations and symptoms that are difficult to accurately distinguish, selecting the appropriate diagnostic techniques for the rapid identification of pathogens is crucial for clinical disease diagnosis and public health management. However, traditional diagnostic techniques have low detection rates, long detection times and limited automation, which means that they do not meet the requirements for rapid diagnosis. Recent years have seen continuous developments in molecular detection technology, which has a higher sensitivity and specificity, shorter detection time and increased automation, and performs an important role in the early and rapid detection of infectious disease pathogens. The present study summarizes recent progress in molecular diagnostic technologies such as PCR, isothermal amplification, gene chips and high‑throughput sequencing for the detection of infectious disease pathogens, and compares the technical principles, advantages and disadvantages, applicability and costs of these diagnostic techniques.
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May-2023
Volume 27 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Liu Q, Jin X, Cheng J, Zhou H, Zhang Y and Dai Y: Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review). Mol Med Rep 27: 104, 2023.
APA
Liu, Q., Jin, X., Cheng, J., Zhou, H., Zhang, Y., & Dai, Y. (2023). Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review). Molecular Medicine Reports, 27, 104. https://doi.org/10.3892/mmr.2023.12991
MLA
Liu, Q., Jin, X., Cheng, J., Zhou, H., Zhang, Y., Dai, Y."Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)". Molecular Medicine Reports 27.5 (2023): 104.
Chicago
Liu, Q., Jin, X., Cheng, J., Zhou, H., Zhang, Y., Dai, Y."Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)". Molecular Medicine Reports 27, no. 5 (2023): 104. https://doi.org/10.3892/mmr.2023.12991