Open Access

In silico and in vitro study of FLT3 inhibitors and their application in acute myeloid leukemia

  • Authors:
    • Ahtziri S. Carranza‑Aranda
    • Luis Felipe Jave‑Suárez
    • Flor Y. Flores‑hernández
    • María Del Rosario Huizar‑López
    • Sara E. Herrera‑Rodríguez
    • Anne Santerre
  • View Affiliations

  • Published online on: October 4, 2024     https://doi.org/10.3892/mmr.2024.13353
  • Article Number: 229
  • Copyright: © Carranza‑Aranda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myeloid leukemia (AML) is the most common hematological cancer in the adult population worldwide. Approximately 35% of patients with AML present internal tandem duplication (ITD) mutations in the FMS‑like tyrosine kinase 3 (FLT3) receptor associated with poor prognosis, and thus, this receptor is a relevant target for potential therapeutics. Tyrosine kinase inhibitors (TKIs) are used to treat AML; however, their molecular interactions and effects on leukemic cells are poorly understood. The present study aimed to gain insights into the molecular interactions and affinity forces of four TKI drugs (sorafenib, midostaurin, gilteritinib and quizartinib) with the wild‑type (WT)‑FLT3 and ITD‑mutated (ITD‑FLT3) structural models of FLT3, in its inactive aspartic acid‑phenylalanine‑glycine motif (DFG‑out) and active aspartic acid‑phenylalanine‑glycine motif (DFG‑in) conformations. Furthermore, the present study evaluated the effects of the second‑generation TKIs gilteritinib and quizartinib on cancer cell viability, apoptosis and proliferation in the MV4‑11 (ITD‑FLT3) and HL60 (WT‑FLT3) AML cell lines. Peripheral blood mononuclear cells (PBMCs) from a healthy volunteer were included as an FLT3‑negative group. Molecular docking analysis indicated higher affinities of second‑generation TKIs for WT‑FLT3/DFG‑out and WT‑FLT3/DFG‑in compared with those of the first‑generation TKIs. However, the ITD mutation changed the affinity of all TKIs. The in vitro data supported the in silico predictions: MV4‑11 cells presented high selective sensibility to gilteritinib and quizartinib compared with the HL60 cells, whereas the drugs had no effect on PBMCs. Thus, the current study presented novel information about molecular interactions between the FLT3 receptors (WT or ITD‑mutated) and some of their inhibitors. It also paves the way for the search for novel inhibitory molecules with potential use against AML.
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December-2024
Volume 30 Issue 6

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Spandidos Publications style
Carranza‑Aranda AS, Jave‑Suárez LF, Flores‑hernández FY, Huizar‑López M, Herrera‑Rodríguez SE and Santerre A: <em>In silico</em> and<em> in vitro</em> study of FLT3 inhibitors and their application in acute myeloid leukemia. Mol Med Rep 30: 229, 2024.
APA
Carranza‑Aranda, A.S., Jave‑Suárez, L.F., Flores‑hernández, F.Y., Huizar‑López, M., Herrera‑Rodríguez, S.E., & Santerre, A. (2024). <em>In silico</em> and<em> in vitro</em> study of FLT3 inhibitors and their application in acute myeloid leukemia. Molecular Medicine Reports, 30, 229. https://doi.org/10.3892/mmr.2024.13353
MLA
Carranza‑Aranda, A. S., Jave‑Suárez, L. F., Flores‑hernández, F. Y., Huizar‑López, M., Herrera‑Rodríguez, S. E., Santerre, A."<em>In silico</em> and<em> in vitro</em> study of FLT3 inhibitors and their application in acute myeloid leukemia". Molecular Medicine Reports 30.6 (2024): 229.
Chicago
Carranza‑Aranda, A. S., Jave‑Suárez, L. F., Flores‑hernández, F. Y., Huizar‑López, M., Herrera‑Rodríguez, S. E., Santerre, A."<em>In silico</em> and<em> in vitro</em> study of FLT3 inhibitors and their application in acute myeloid leukemia". Molecular Medicine Reports 30, no. 6 (2024): 229. https://doi.org/10.3892/mmr.2024.13353