MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma

  • Authors:
    • Yoichiro Mori
    • Hideyuki Ishiguro
    • Yoshiyuki Kuwabara
    • Masahiro Kimura
    • Akira Mitsui
    • Ryo Ogawa
    • Takeyasu Katada
    • Koshiro Harata
    • Tatsuya Tanaka
    • Midori Shiozaki
    • Yoshitaka Fujii
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/mmr_00000089
  • Pages: 235-239
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Abstract

It has been suggested that microRNA-21 (miR-21) functions as an oncogene, as it is overexpressed in many types of tumors compared to adjacent normal tissues. However, the role of miR-21 has yet to be studied in esophageal squamous cell carcinoma (ESCC). miR-21 expression was quantified by real-time reverse transcription polymerase chain reaction in 38 ESCC specimens and their paired non-cancerous mucosa, and in 15 esophageal cancer cell lines (TE1-15). miR-21 expression levels in ESCC tissue were significantly higher than in the corresponding non-cancerous mucosa (6.873±12.664 vs. 1.000, p<0.0001). In patients with more advanced (T3 or T4) tumors, miR-21 expression levels were significantly higher than in those with less advanced (T1 or T2) tumors (P=0.0333). miR-21 expression levels in patients with more invasive infiltrative growth pattern (inf) β tumors were significantly higher than in patients with less invasive infα tumors (P=0.0166). Among the cell lines studied, TE9 had the lowest and TE1 the highest expression of miR-21. Using the miRNA precursor or antisense miRNA inhibitor, we studied how the level of miR-21 influences the proliferation of ESCC cells. Cell proliferation of the anti-miR-21-transfected cell line was significantly lower, while that of the pre-miR-21-transfected cell line was significantly higher than in the control. In ESCC, miR-21 expression may be involved in tumor growth and invasion.

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March-April 2009
Volume 2 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Mori Y, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Ogawa R, Katada T, Harata K, Tanaka T, Shiozaki M, Shiozaki M, et al: MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma. Mol Med Rep 2: 235-239, 2009.
APA
Mori, Y., Ishiguro, H., Kuwabara, Y., Kimura, M., Mitsui, A., Ogawa, R. ... Fujii, Y. (2009). MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma. Molecular Medicine Reports, 2, 235-239. https://doi.org/10.3892/mmr_00000089
MLA
Mori, Y., Ishiguro, H., Kuwabara, Y., Kimura, M., Mitsui, A., Ogawa, R., Katada, T., Harata, K., Tanaka, T., Shiozaki, M., Fujii, Y."MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma". Molecular Medicine Reports 2.2 (2009): 235-239.
Chicago
Mori, Y., Ishiguro, H., Kuwabara, Y., Kimura, M., Mitsui, A., Ogawa, R., Katada, T., Harata, K., Tanaka, T., Shiozaki, M., Fujii, Y."MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma". Molecular Medicine Reports 2, no. 2 (2009): 235-239. https://doi.org/10.3892/mmr_00000089