Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model
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- Published online on: February 13, 2017 https://doi.org/10.3892/ol.2017.5731
- Pages: 2323-2329
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Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Granulocyte colony-stimulating factor (G‑CSF) mobilizes regulatory T cells (Tregs) from bone marrow into the peripheral blood, by reducing the expression of stromal cell‑derived factor‑1α (SDF-1α). However, G‑CSF has rarely been studied in acute myeloid leukemia (AML) immunotherapy. The present study performed a Transwell migration assay in vitro to determine the contribution of SDF‑1α to the migration of leukemia cells, and the effects of G‑CSF were evaluated. The effects of G‑CSF on SDF‑1α and Tregs in the AML microenvironment were examined, by employing a WEHI‑3‑grafted BALB/c mouse AML model (AML-M4). It is evident that G-CSF reversed immunosuppression of the AML microenvironment by reducing SDF‑1α in bone marrow and elevating Tregs in the peripheral blood in in vivo studies. Furthermore, AML mice treated with vaccines combined with G‑CSF achieved a longer survival time than those treated with vaccines without G‑CSF, showing the efficiency of the regimen. The present study demonstrates the effects of G‑CSF on the mobilization of leukemia cells and Tregs into the peripheral blood. In addition, immunotherapy with G-CSF priming represents a promising therapeutic strategy of targeting the immunosuppression.
