Open Access

Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model

  • Authors:
    • Jinqiu Chen
    • Miling Zhang
    • Fuling Zhou
    • Jin Wang
    • Ben Niu
    • Wanggang Zhang
  • View Affiliations

  • Published online on: February 13, 2017     https://doi.org/10.3892/ol.2017.5731
  • Pages: 2323-2329
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Granulocyte colony-stimulating factor (G‑CSF) mobilizes regulatory T cells (Tregs) from bone marrow into the peripheral blood, by reducing the expression of stromal cell‑derived factor‑1α (SDF-1α). However, G‑CSF has rarely been studied in acute myeloid leukemia (AML) immunotherapy. The present study performed a Transwell migration assay in vitro to determine the contribution of SDF‑1α to the migration of leukemia cells, and the effects of G‑CSF were evaluated. The effects of G‑CSF on SDF‑1α and Tregs in the AML microenvironment were examined, by employing a WEHI‑3‑grafted BALB/c mouse AML model (AML-M4). It is evident that G-CSF reversed immunosuppression of the AML microenvironment by reducing SDF‑1α in bone marrow and elevating Tregs in the peripheral blood in in vivo studies. Furthermore, AML mice treated with vaccines combined with G‑CSF achieved a longer survival time than those treated with vaccines without G‑CSF, showing the efficiency of the regimen. The present study demonstrates the effects of G‑CSF on the mobilization of leukemia cells and Tregs into the peripheral blood. In addition, immunotherapy with G-CSF priming represents a promising therapeutic strategy of targeting the immunosuppression.

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April-2017
Volume 13 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen J, Zhang M, Zhou F, Wang J, Niu B and Zhang W: Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model. Oncol Lett 13: 2323-2329, 2017
APA
Chen, J., Zhang, M., Zhou, F., Wang, J., Niu, B., & Zhang, W. (2017). Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model. Oncology Letters, 13, 2323-2329. https://doi.org/10.3892/ol.2017.5731
MLA
Chen, J., Zhang, M., Zhou, F., Wang, J., Niu, B., Zhang, W."Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model". Oncology Letters 13.4 (2017): 2323-2329.
Chicago
Chen, J., Zhang, M., Zhou, F., Wang, J., Niu, B., Zhang, W."Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model". Oncology Letters 13, no. 4 (2017): 2323-2329. https://doi.org/10.3892/ol.2017.5731