Mastl overexpression is associated with epithelial to mesenchymal transition and predicts a poor clinical outcome in gastric cancer
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- Published online on: October 10, 2017 https://doi.org/10.3892/ol.2017.7155
- Pages: 7283-7287
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Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Gastric carcinoma remains one of the most common and lethal types of malignancy worldwide (1). Although patients receive radical surgery and chemotherapy, recurrence and metastasis remain a challenge for this type of malignancy (2). Therefore, the identification of novel therapeutic targets and prognostic factors for patients with gastric cancer is required.
Kinases represent a group of promising therapeutic targets for cancers. Identification of novel specific kinase targets and prognostic factors may not only expand upon the options of treatments for patients with gastric cancer, but also provide classifiers to discriminate subgroups that may benefit from kinase inhibitors (3).
The microtubule-associated serine/threonine kinase like (Mastl) gene encodes a kinase that promotes mitotic progression and cell cycle re-entry following DNA damage (4,5). A previous study demonstrated that Mastl was commonly overexpressed in a number of types of cancer, including breast and oral cancer (6). Furthermore, Mastl upregulation was associated with recurrence following initial cancer therapy and decreased patient survival in a number of cancer types (6,7). These previous studies indicated that Mastl may be a novel therapeutic target for cancer. In spite of this, the expression of Mastl in patients with gastric cancer has not been investigated and limited information is known about the mechanisms of Mastl with respect to tumor progression. In the present study, it was hypothesized that Mastl may induce epithelial to mesenchymal transition (EMT) and consequently promote tumor migration and metastasis. Therefore, the present study aimed to investigate the expression level of Mastl and EMT-associated proteins in a cohort of patients with gastric cancer, and to evaluate the associated clinical significance and correlation.
Materials and methods
Patients and tissue specimens
The present study was approved by the Ethics Committee of Shandong Cancer Hospital and Institute (Jinan, China). A cohort of 152 tumor node metastasis (TNM) (8) stage II/III patients with gastric cancer (84 male, 68 female; median age 52 years, range 28–75 years), who underwent gastrectomy with lymph node dissection between July 2006 and December 2010, were included in this retrospective study. TNM staging, histology and margins were classified or defined according to the Chinese guidelines for diagnosis and treatment of gastric cancer (2011 edition) (8). The following exclusion criteria were predefined: i) Patients with R1/R2 margins (as defined by the aforementioned guidelines); ii) patients who succumbed during the peri-operative period; and iii) patients receiving preoperative chemotherapy or radiotherapy. Finally, a total of 126 patients with advanced disease (where metastasis or recurrence occurred during the follow-up period) received postoperative platinum-based chemotherapy with or without radiotherapy were included in the present study following the exclusion of 26 cases. All patients were followed up subsequent to surgery. Examinations for recurrence or metastasis were performed using serum tumor marker assays, including carcinoembryonic antigen, and diagnostic imaging (computed tomography, ultrasonography or magnetic resonance imaging) every 3 months for the initial 2 postoperative years and subsequently every 6 months. The median follow-up time was 60 months (range, 1–111 months). Patients who experienced distant metastases or recurrence during follow-up received oxaliplatin (85 mg/m2, every 2 weeks), docetaxel (75 mg/m2, every 3 weeks) or irinotecan (150–180 mg/m2, every 2 weeks) single agent chemotherapy, or treatment in combination with cetuximab (500 mg/m2, every 2 weeks) or bevacizumab (5 mg/kg, every 2 weeks) regimens (8).
Immunohistochemistry (IHC)
Immunohistochemical staining was performed to determine the protein expression of Mastl, Vimentin and epithelial (E-)cadherin in 126 gastric cancer tissues. Briefly, 4-µm 10% formalin-fixed (1 day at room temperature) paraffin embedded sections were dewaxed in xylene and antigen was retrieved by heating the sections (95°C for 30 min) in 10 mmol/l citrate solution (pH 6.0) for 30 min. After blocking the endogenous peroxidase activity, using 3% H2O2 (10 min at room temperature), anti-Mastl (#15739-1-AP, ProteinTech Group, Inc., Chicago, IL, USA), anti-Vimentin (#10366-1-AP, ProteinTech Group, Inc.) and anti-E-Cadherin (#20874-1-AP, ProteinTech Group, Inc.) (all dilution at 1:100) antibodies were incubated with the sections overnight at 4°C. Subsequently, sections were washed and treated with biotinylated horseradish peroxidase-labeled anti rabbit secondary antibody (#sc-2357, Santa Cruz Biotechnology, Inc., Dallas, TX, USA, 1:100) at 37°C for 30 min. Diaminobenzene was used to detect chromogen and hematoxylin was used as the nuclear counterstain at room temperature for 1 min, and staining was observed under light microscope (magnification, ×40). Non-specific rabbit immunoglobulin G (#sc-2027, at 1:100 dilution, Santa Cruz Biotechnology, Inc.) was used to replace the primary antibodies in immunohistostaining as negative controls.
Evaluation of immunohistochemical staining
The immunostaining was scored by two independent senior pathologists who were unaware of the patients' clinical information. For Mastl staining, an immunoreactive score (IRS) was derived by addition of the intensity score (0, negative to weak; 1, positive; and 2, strongly positive) and the density score (0, 0–25% of tumor area stained; 1, 26–50; 2, 51–75; and 3, 76–100%) for statistical analysis with respect to each patient. An IRS score of ≥4 was defined as high expression and <4 was defined as low expression, as described previously (9). The membranous E-cadherin and cytoplasmic vimentin expression was defined according to previously published guidelines (10). The slides were graded as follows: Grade 1, 0–25% staining; grade 2, 26–50% staining; and grade 3, >50% staining. According to previous criteria (11), patients with vimentin or E-cadherin expression of greater than or equal to grade 1 were classified as exhibiting positive EMT status, while the other patients were defined as the wild-type group.
Statistical analysis
The χ2 test or Fisher's exact test was used to evaluate the association between Mastl expression, the clinicopathological variables and EMT status. Kaplan-Meier estimator curves were plotted using a log-rank test for univariable analysis of overall survival and differences between the groups were evaluated using the log-rank test. Multivariable Cox's proportional hazards regression models were used to assess the prognostic significance of Mastl expression and of several accepted prognostic factors. Statistical analysis was performed using the GraphPad Prism 5.0 software package (GraphPad Software, Inc., San Diego, USA). P<0.05 was considered to indicate a statistically significant difference.
Results
Table I lists the clinicopathological characteristics of the 126 patients with gastric cancer patients included in the present study. First, the Mastl expression level in gastric cancer and adjacent normal gastric tissues was analyzed using IHC. The representative images of immunohistochemical staining are presented in Fig. 1. Mastl immunoreactivity was primarily within the membranes and cytoplasm of the gastric cancer cells. The majority of the stroma or normal gastric cells exhibited negative staining, in spite of sporadic positive staining on these cells. According to the IHC analysis, the patients were separated into two groups: High Mastl expression (IRS≥4) and low Mastl expression (IRS<4). Furthermore, 54/126 (42.9%) cases exhibited high expression of Mastl in the gastric cancer tissues.
The association between Mastl protein expression and the clinicopathological characteristics of gastric cancer was subsequently determined. This analysis revealed that increased expression of Mastl was positively associated with lymph node metastasis (P=0.013) and tumor recurrence (P=0.016). However, no association between Mastl expression and other clinicopathological features was observed (Table I).
As seen in Fig. 1, Positive E-cadherin staining was observed in normal and part of the malignant gastric epithelium, and vimentin staining was observed in all the stroma and part of the cancerous epithelium. On the basis of the IHC results of Vimentin and E-cadherin and the aforementioned previously published criteria (11), the patients were separated into two groups: EMT type and wild-type. A total of 31/126 (24.6%) cases were defined as the EMT type in the gastric cancer cohort. Increased Mastl expression was identified to be significantly associated with EMT status (P=0.029).
The patients with high Mastl expression exhibited a significantly decreased overall survival (OS) time compared with that of the patients with low Mastl expression [hazard ratio (HR), 2.591; 95% confidence interval (CI), 1.535–4.374; log-rank test, P<0.001] (Fig. 2; Table II). Furthermore, a survival analysis was performed according to the level of Mastl expression in subsets of patients with gastric cancer at different clinical stages. The results demonstrated that high expression of Mastl was a prognostic factor for the patients with stage III disease (HR, 2.590; 95% CI, 1.355–4.953; log-rank test, P=0.0045) (Fig. 2). However, Mastl expression levels failed to retain prognostic significance for the patients at stage II (HR, 2.1810; 95% CI, 0.8943–5.3190; log-rank test, P=0.0503) (Fig. 2).
Table II.Prognostic factors on univariable and multivariable Cox proportional hazards regression models for overall survival in stage II and III colorectal cancer patients. |
Clinical characteristics were included in the univariate analysis to assess their impact on the survival of patients with gastric cancer; positive lymph node metastasis, underlying EMT status and high Mastl expression were identified to be prognostic factors, but were not associated with each other. On multivariable analysis adjusted for established clinical prognostic factors, high Mastl expression was a prognostic factor of OS time (HR, 2.006; 95% CI, 1.176–3.420; P=0.010), as was the presence of lymph node metastasis (HR, 1.681; 95% CI, 1.130–2.501; P=0.011) and EMT status (HR, 2.605; 95% CI, 1.533–4.426; P<0.001).
Discussion
The clinical significance of Mastl expression has been investigated only in oral and breast cancer (6). To the best of our knowledge, the present study was the first to clinically evaluate the expression of Mast1 and its significance in gastric cancer. The results of the present study identified that Mastl protein was upregulated in the gastric cancer tissues, relative to the normal adjacent non-cancerous tissues, which was consistent with the results of a previous study of oral cancer tissues and healthy controls (6). Furthermore, analysis of gastric cancer tissues revealed a significant association between Mastl expression with aggressive characteristics and poor patient survival, which is consistent with the results of a previous study of breast cancer (7). Therefore, the present study revealed Mastl as a candidate oncogene and a potential prognostic factor in cancer, and Mastl-positive gastric cancer may require intensive treatments.
Mastl-knockdown in recurrent tumor cells may re-sensitize their response to chemotherapy and radiotherapy in vitro and in vivo, indicating that Mastl is a novel therapeutic target for tumor recurrence (6). In vitro studies have indicated a number of possible mechanisms for this effect, including Mastl acting as a cyclin-dependent kinase dephosphorylation inhibitor and a regulator of the DNA damage response (12,5). However, the association between Mastl and tumor recurrence remains unknown. In the present study, it was validated that, in gastric cancer samples, Mastl was associated with EMT status. Previous studies have identified that EMT is involved in tumor recurrence and the resistance to initial cancer therapy (13,14), and the results of the present study support this.
Polo-like kinase 1 (Plk1), aurora kinases and Mastl represent a group of promising serine/threonine kinase targets for cancer treatment. Inhibitors of Plk1 and aurora kinases are currently under clinical development for cancer therapy (15,16). On the basis of the biological and the clinical significance of Mastl in the present and previous studies, it has been hypothesized that Mastl may sensitize the response to initial cancer therapy in gastric cancer, thus decreasing the incidence of tumor recurrence. Additionally, potential Mast1 inhibitors deserve future investigation.
To the best of our knowledge, the results of the present study were the first to indicate that Mastl expression is associated with EMT status, tumor recurrence and poor survival in gastric cancer. The expression of Mastl, as determined using IHC, may serve as an independent predictor of clinical outcome for patients with gastric cancer. The results of the present study suggested that Mastl may serve as a promising therapeutic target for gastric cancer. Additional studies are required to investigate the clinical potential of Mastl inhibition for gastric cancer therapy.
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