This article is mentioned in:

Introduction

The incidence of cervical cancer (CC) (1) ranks second among female malignant tumors worldwide. As one of the most common malignant tumors in gynecology, the most important risk factor for it (2) is the persistent infection of high-risk HPV that involves more than 89% of CC patients. Due to the high recurrence rate of high-risk HPV infection, CC patients in advanced stage have a poor prognosis with a high mortality. The early diagnosis and accurate analysis of CC are important for the treatment effect and prognosis of patients. At this stage, CC is diagnosed mainly by colposcopy, cervical and cervical tube biopsy, cervical cell scraping plate and high-risk HPV detection (3). Combined with the comprehensive clinical situation of CC patients, the clinical staging of CC was performed on them. The treatment methods and patient prognosis of different clinical stages of it are also different (4). With the continuous development and improvement of imaging technology in recent years, ultrasonic elastography technology has a good performance in the early diagnosis and pathological staging of CC, with its non-invasive and repeatability favored by clinics and patients (5).

Ultrasonic elastography (6) is a new type of ultrasonic diagnostic technology. It uses the difference of elasticity coefficient between different biological tumor tissues or other lesion areas and surrounding normal tissues to generate difference in strain size. It also uses multi-color coding to display images, so as to determine the elasticity of lesion tissues, thereby analyzing the possibility of canceration. At present, ultrasonic elastography has been used in the clinical application of prostate cancer, thyroid cancer, breast cancer and early CC (7). In this study, ultrasonic elastography in the diagnosis of the clinical staging of CC and its evaluation value of the treatment effect of positive CC were investigated.

Materials and methods

Patient information

A total of 160 suspected CC patients treated in Zhengzhou Central Hospital Affiliated to Zhengzhou University (Zhengzhou, China) (pathologically examined and 75 patients diagnosed as CC) were included, aged 25–68 years old, with an average age of 50.7±8.4 years (Table I).

Table I. Clinical information sheet.

Table I.

Clinical information sheet.

Factors	n (%)
Age
<50	65 (40.63)
≥50	95 (59.37)
Married
Yes	143 (89.38)
No	17 (10.62)
Pregnant
Yes	125 (78.13)
No	35 (21.87)
Smoking
Yes	89 (55.63)
No	71 (44.37)
FIGO staging of positive CC (n=75)
Stage I	26 (34.67)
Stage II	14 (18.67)
Stage III	19 (25.33)
Stage IV	16 (21.33)
Pathological diagnosis typing
Squamous cell carcinoma	48 (30.00)
Adenocarcinoma	67 (41.87)
Adenosquamous carcinoma	45 (28.13)

Inclusion and exclusion criteria

Only CC patients treated in Zhengzhou Central Hospital Affiliated to Zhengzhou University were included. The inclusion criteria of all CC patients were in line with the diagnostic criteria of international CC.

Patients with various family hereditary diseases were excluded and patients with cancer diseases other than CC, were excluded.

This study was approved by the Ethics Committee of Zhengzhou Central Hospital Affiliated to Zhengzhou University. Patients who participated in this research had complete clinical data. The signed informed consents were obtained from the patients or the guardians.

Conventional vaginal ultrasound

The Hitachi Hi Vision 900 Color Doppler Ultrasound Diagnostic Apparatus (Hitachi, Ltd., Tokyo, Japan) was used. The patient was asked to drink 500 ml of water and suppress the urine before examination, taking the supine position. Conventional abdominal gynecological ultrasound was performed before angiography. A conventional ultrasound diagnosis was performed after the general condition of the uterus and accessories recorded. After the rapid injection of ultrasonic contrast agent through the elbow vein, images from 120 sec-3 min were continuously stored and observed. Ultrasonic image findings of the specific clinical staging of CC are shown in Table II.

Table II. Ultrasonic image findings of different FIGO stages of CC.

Table II.

Ultrasonic image findings of different FIGO stages of CC.

Groups	Image findings
Stage I	The cancer site is only in the cervix
Stage II	The cancer site has gone beyond the cervix but not reached the lower third of the vagina or the pelvic wall
Stage III	The cancer site has invaded the pelvic wall or has spread to the lower third of the vagina
Stage IV	The cancer site has infiltrated the rectal mucosa or bladder, beyond the true pelvis

Elastic ultrasound

The Hitachi Hi Vision 900 Color Doppler Ultrasound Diagnostic Apparatus (Hitachi, Ltd.) was used, equipped with tissue elastography software. A real-time elastography examination was performed after conventional vaginal ultrasound. The region of interest (ROI) of the CC patient's cancer mass was moved to the center of the image, so that the sampling frame size was more than twice the lesion size. The double-elastography mode was used for the simultaneous display of two-dimensional images and real-time elastography. The probe force was slowly controlled to pressurize to touch the cervix, with a pressure touch frequency of 2–4 times/sec. When the pressure curve was displayed, it was expressed as the comprehensive indicator of the pressure release frequency and pressure. At this time, the pressure touch frequency should be maintained between the comprehensive indicator of 3–4 levels, so as to maintain the stability between the pressure curve and the image for 5–6 sec. The change of the cancer tissue color in the sampling frame was observed. The elastography of the cancer site was obtained after the image was stable displayed. The average hardness of the tissue in the ROI was represented by green, that higher than the average hardness by blue, and that relatively low by red. The ultrasonic elastography of all CC patients was performed independently by three imaging physicians with more than 4 years of experience in elastic ultrasound.

Efficacy evaluation

Based on the World Health Organization (WHO) solid tumor clinical treatment evaluation criteria, the clinical efficacy of positive CC was divided into four parts. After treatment, the target lesion diameter increases by ≥50% compared with before treatment and new lesions are produced as progression of disease (PD). After treatment, the target lesion diameter is gradually reduced, with a reduction ratio of >50% compared with before treatment, the duration is >4 weeks, and no new lesion is produced as partial remission (PR). After treatment, the target lesion diameter is gradually reduced, with a reduction ratio of <50% compared with before treatment, and no new lesion is produced as stable disease (SD). The target lesion continues to disappear for >4 weeks and no new lesion is produced as complete remission (CR). Clinical overall efficiency OR = (CR + PR)/the total number of cases × 100%.

Elastic ultrasound SR evaluation refers to relevant international reports on changes in the SR value of static ROI before and after CC radiotherapy. CR refers to patients with the disappearance of lesions after radiotherapy. PR refers to patients with the SR value decreasing to 81% before radiotherapy. SD refers to patients with the change in the SR value from 8l to 102% before treatment. PD refers to patients with the SR value increasing to more than 102% before radiotherapy. Overall efficiency of SR OR = (CR + PR)/the total number of cases × 100%.

Statistical analysis

SPSS17.0 software (SPSS, Inc., Chicago, IL, USA) was used for statistical analysis. Count data were expressed as (%). t-test was used for the comparison between the two methods. When the test results shows P<0.05, there is a significant difference in the comparison between the two, and the difference is statistically significant.

Results

Comparison of results between conventional ultrasound and ultrasonic elastography in pathological diagnosis of CC

The results of conventional ultrasound in the diagnosis of 160 CC patients were: Αmong 75 positive CC patients diagnosed by pathology, 54 cases of positive CC were diagnosed by conventional ultrasound with a sensitivity of 72.00% (54/75), and 71 cases diagnosed by ultrasonic elastography with a sensitivity of 94.67% (71/75). The sensitivity of ultrasonic elastography was significantly higher than that of conventional ultrasound, with a statistically significant difference (P<0.001). Among 85 negative CC patients diagnosed by pathology, 60 cases were diagnosed by conventional ultrasound with a specificity of 70.59% (60/85), and 79 cases diagnosed by ultrasonic elastography with a specificity of 92.94% (79/85). The specificity of ultrasonic elastography was significantly higher than that of conventional ultrasound, with a statistically significant difference (P<0.001). The accordance rate of conventional ultrasound for the clinical diagnosis of CC was 71.25% (114/160), and that of ultrasonic elastography was 93.75% (150/160). The accordance rate of ultrasonic elastography was significantly higher than that of conventional ultrasound, with a statistically significant difference (P<0.001) (Tables III–V).

Table III. Results of conventional ultrasound in pathological diagnosis of CC.

Table III.

Results of conventional ultrasound in pathological diagnosis of CC.

	Results of pathological diagnosis
Conventional ultrasound	Positive	Negative	Total
Positive	54	25	79
Negative	21	60	81
Total	75	85	160

Table V. Comparison of results between conventional ultrasound and ultrasonic elastography in pathological diagnosis of CC.

Table V.

Comparison of results between conventional ultrasound and ultrasonic elastography in pathological diagnosis of CC.

Factors	Conventional ultrasound	Ultrasonic elastography	χ2	P-value
Sensitivity	72.00% (54/75)	94.67% (71/75)	13.870	<0.001
Specificity	70.59% (60/85)	92.94% (79/85)	14.240	<0.001
Diagnostic accordance rate	71.25% (114/160)	93.75% (150/160)	28.050	<0.001

Comparison of results between conventional ultrasound and ultrasonic elastography in diagnosis of 75 cases of positive CC in clinical stage III and IV

The sensitivity, specificity and diagnostic accordance rate of ultrasonic elastography in the pathological diagnosis of CC in stage III were 94.74, 96.43 and 96.00%, respectively, higher than those of conventional ultrasound, which were 68.42, 91.07 and 85.33%, respectively. Except for the specificity, the other two P-values were <0.05, with a statistically significant difference. The sensitivity, specificity and diagnostic accordance rate in stage IV were 87.50, 98.31 and 96.00%, respectively, higher than those of conventional ultrasound, which were 56.25, 89.83 and 82.66%, respectively. Except for the specificity, the other two P-values were <0.05, with a statistically significant difference (Tables VI-X).

Table VI. Accuracy of conventional ultrasound in diagnosis of CC patients in stage III

Table VI.

Accuracy of conventional ultrasound in diagnosis of CC patients in stage III

	Results of pathological diagnosis
Conventional ultrasound	Stage III	Non-stage III	Total
Stage III	13	5	18
Non-stage III	6	51	57
Total	19	56	75

Table X. Comparison of results between conventional ultrasound and ultrasonic elastography in diagnosis of 75 cases of positive CC in clinical stage III and IV.

Table X.

Comparison of results between conventional ultrasound and ultrasonic elastography in diagnosis of 75 cases of positive CC in clinical stage III and IV.

Factors	Conventional ultrasound	Ultrasonic elastography	χ2	P-value
Sensitivity
Stage III	68.42% (13/19)	94.74% (18/19)	4.378	0.036
Stage IV	56.25% (9/16)	87.50% (14/16)	3.865	0.049
Specificity
Stage III	91.07% (51/56)	96.43% (54/56)	1.371	0.242
Stage IV	89.83% (53/59)	98.31% (58/59)	3.797	0.050
Diagnostic accordance rate
Stage III	85.33% (64/75)	96.00% (72/75)	5.042	0.025
Stage IV	82.66% (62/75)	96.00% (72/75)	6.996	0.008

Comparison of evaluation value between conventional ultrasound and elastic ultrasound SR value in radiotherapy efficacy in CC patients

The sensitivity, specificity and diagnostic accordance rate of conventional ultrasound for the efficacy evaluation of radiotherapy in 75 positive CC patients were 63.64, 66.67 and 64.00%, respectively, lower than those of elastic ultrasound SR value, which were 86.36, 77.78 and 85.33%, respectively. Except for the specificity, the other two P-values were <0.05, with a statistically significant difference (Tables XI-XIII).

Table XI. Evaluation value of conventional ultrasound in radiotherapy efficacy in positive CC patients.

Table XI.

Evaluation value of conventional ultrasound in radiotherapy efficacy in positive CC patients.

	Evaluation of radiotherapy efficacy
Conventional ultrasound	Effective (PR+CR)	Ineffective (SD+PD)	Total
Effective	42	3	45
Ineffective	24	6	30
Total	66	9	75

Table XIII. Comparison of value between conventional ultrasound and elastic ultrasound SR value in radiotherapy efficacy in positive CC patients.

Table XIII.

Comparison of value between conventional ultrasound and elastic ultrasound SR value in radiotherapy efficacy in positive CC patients.

Factors	Conventional ultrasound	Ultrasonic elastography	χ2	P-value
Sensitivity	63.64% (42/66)	86.36% (57/66)	9.091	0.003
Specificity	66.67% (6/9)	77.78% (7/9)	0.277	0.599
Diagnostic accordance rate	64.00% (48/75)	85.33% (64/75)	9.023	0.003

Discussion

Cervical cancer (CC) is a female reproductive system malignancy caused by high-risk HPV, with an incidence second only to breast cancer (8) around the world. The onset symptom of early CC is not very obvious, leading to the development of it to the middle or late stage when visiting a doctor, which has a great impact on the treatment effect of CC. Therefore, CC patients should be early detected and early intervened. The reasonable treatment of them according to the different diagnosis results of different clinical stages of CC is important for their treatment efficacy and prognosis quality (9–11). At present, due to its long inspection time, cumbersome operation process and expensive price, the commonly used CC screening method (12–14) in clinic, such as traditional cervical pathological cell examination, has a certain impact on the patient's body and mind. The carcinogenesis of CC is from early to late stage, from stages I–IV, so pathological section diagnosis is not suitable for the examination of CC stages. In recent years, there has been a new breakthrough in ultrasonic imaging technology. It is found that the installation of unique elastic imaging software on the ultrasound scanner can greatly improve the accuracy rate of common ultrasound technology, so that the clinical application value is higher (15). In this study, the non-invasive ultrasonic elastography technology was used to detect and diagnose CC patients, and to explore its value in the efficacy evaluation of CC. The clinical diagnosis methods of CC are often cervical and cervical tube biopsy (16,17). However, cervical biopsy has limitations in clearly showing the extent of CC violation, but elastic ultrasound can accurately show it, thus inferring the clinical staging of CC. CC staging has important clinical guiding significance for treatment options, surgical methods and the choice of radiotherapy or chemotherapy. The development of new imaging technology has improved the accordance rate of the clinical staging of CC (18).

In this study, the application of ultrasonic elastography technology in the clinical staging of CC was first explored. The result differences between conventional ultrasound and ultrasonic elastography in the pathological diagnosis of CC were compared. It was found that the sensitivity, specificity and diagnostic accordance rate of ultrasonic elastography for CC were significantly higher than those of conventional ultrasound, with a statistically significant difference (P<0.001). The sensitivity and diagnostic accordance rate of ultrasonic elastography in the pathological diagnosis of 75 cases of positive CC in stage III and IV were significantly higher than those of conventional ultrasound, with a statistically significant difference (P<0.05). Therefore, it is believed that ultrasonic elastography has a higher sensitivity, specificity and diagnostic accordance rate for the diagnosis of the clinical staging of CC. This is similar to the research viewpoints of Fischerova et al (19). They used the latest elastic ultrasound technology to determine the elastic strain ratio and elastic image pressure release indicator of CC patients and healthy control group, so as to diagnose the tumor staging and incidence of CC patients. The results showed that elastic ultrasound technology, which can be used as an effective diagnosis method of CC, had an extremely high diagnostic accordance rate for CC pathological patients. Its elastic strain ratio had a certain direct correlation with the onset degree of CC. After that, the value differences between conventional ultrasound and elastic ultrasound SR value in the efficacy of radiotherapy in positive CC patients were compared. The results showed that the sensitivity, specificity and diagnostic accordance rate of elastic ultrasound SR value in the efficacy evaluation of radiotherapy in positive CC patients were higher than those of conventional ultrasound. Except for the specificity, the other two P-values were <0.05, with a statistically significant difference. In view of the application value of ultrasonic elastography in tumor efficacy, early researches (20) have confirmed that ultrasonic elastography technology and the clinical efficacy evaluation of tumor have a very high test consistency.

In this experiment, the number of CC cases collected was insufficient, with only 160 cases studied. It cannot be studied as big data, which may result in some contingency in experimental results. The image scanning instrument used this time was not the most advanced, causing some deviations in result analysis. Nevertheless, the error caused by all human factors was avoided as much as possible.

In summary, ultrasonic elastography has a very high application value in the diagnosis of the clinical staging of CC and in clinical efficacy evaluation. With non-invasive and high soft tissue resolution, clearly displaying the structure of various layers of the cervix, it can determine the lesion site, the extent of tumor infiltration into the vagina or uterus and the para-uterine metastasis, thereby improving the accuracy rate of clinical staging. In recent years, elastic ultrasound technology has been greatly developed (21), which can be widely promoted in clinic, so as to improve the diagnostic accuracy rate of the preoperative staging of CC and guide clinical treatment.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

YZ wrote the manuscript. YZ and YYang were responsible for conventional vaginal ultrasound data analysis. YYan and YYang contributed to elastic ultrasound data analysis. All authors read and approved the final manuscript.

Ethics approval and consent to participate

This study was approved by the Ethics Committee of Zhengzhou Central Hospital Affiliated to Zhengzhou University (Zhengzhou, China). Patients who participated in this research had complete clinical data. The signed informed consents were obtained from the patients or the guardians.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References