hsa_circ_0004846 enhances the malignant phenotype of papillary thyroid carcinoma cells via the miR‑142‑3p/PELI1 axis

Ding,Xiaojie; Li,Ruiqi; Xu,Jingya; Hu,Guangquan; Wang,Wenping; Lv,Qihuan; Wang,Youmin

doi:10.3892/ol.2025.14949

hsa_circ_0004846 enhances the malignant phenotype of papillary thyroid carcinoma cells via the miR‑142‑3p/PELI1 axis

Authors:
- Xiaojie Ding
- Ruiqi Li
- Jingya Xu
- Guangquan Hu
- Wenping Wang
- Qihuan Lv
- Youmin Wang
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Affiliations: Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Cardiology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Endocrinology, Anhui No. 2 Provincial People's Hospital, Hefei, Anhui 230041, P.R. China, Department of International Medical Service, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui 230001, P.R. China
Published online on: February 27, 2025 https://doi.org/10.3892/ol.2025.14949
Article Number: 203
Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) are closely associated with human tumorigenesis; however, whether hsa_circ_0004846 serves a role in the progression of papillary thyroid carcinoma (PTC) remains unclear. Therefore, the present study aimed to investigate the effect of hsa_circ_0004846 on PTC. The results demonstrated that circ_0004846 was abnormally upregulated in PTC tissues and thyroid cancer cell lines (BCPAP, TPC‑1 and IHH‑4). Furthermore, hsa_circ_0004846‑overexpressing or ‑depleted PTC cell lines (TPC‑1 and IHH‑4) were constructed using a lentiviral vector system. Notably, hsa_circ_0004846 overexpression markedly promoted cell proliferation, migration and invasion, as evidenced by activation of the PI3K/AKT pathway and the upregulation of vimentin, a class‑III intermediate filament, which acts by regulating cell attachment and migration. However, hsa_circ_0004846 knockdown displayed the opposite effects. Mechanistically, the regulatory association among hsa_circ_0004846, microRNA (miR)‑142‑3p and Pellino E3 ubiquitin protein ligase 1 (PELI1) was validated using a dual‑luciferase reporter assay. Specifically, the results demonstrated that hsa_circ_0004846 could sponge miR‑142‑3p, and the expression levels of miR‑142‑3p were negatively associated with those of hsa_circ_0004846. In addition, PELI1, a cancer‑related E3 ubiquitin ligase, was identified as a downstream target of the hsa_circ_0004846/miR‑142‑3p axis in PTC. Therefore, PELI1 silencing could reverse the hsa_circ_0004846‑induced malignant phenotype of PTC cells. Taken together, the results of the current study highlighted the effect of the hsa_circ_0004846/miR‑142‑3p/PELI1 regulatory network on PTC progression, thus providing a promising target for PTC treatment.

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