Serum HBsAg or anti-HCV were identified in over 90% of hepatocellular carcinoma (HCC) patients in Japan with positive rates approximately 20% and 70%, respectively. In this study, we detected HBV DNA and HCV RNA sequences in tumor or non-tumor tissues obtained from 16 hepatectomized HCC cases, using the polymerase chain reaction (PCR) method for HBV x gene and reverse transcription (RT)-PCR for HCV RNA. Only 3 cases were positive of HBsAg in serum, however, HBV x gene could be detected in the tumor or non-tumor tissues of 13 cases among 16 HCC patients. In two cases, HBV x gene was detected exclusively in tumor or non-tumor tissues. Whereas HCV RNA was detected in both tumor and non-tumor in 9 cases, which results coincided with the anti-HCV testing in sera. These results indicate that HBV x gene might be detected as the integrated form in the liver genomic DNA, while HCV RNA as viremia. The frequent detection of HBV DNA suggests that HBV may be associated with the development of HCC predominantly or cooperatively with HCV.

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