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Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells

  • Authors:
    • Yue Aratanechemuge
    • Hiroshige Hibasami
    • Koh Sanpin
    • Hirotaka Katsuzaki
    • Kunio Imai
    • Takashi Komiya

  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/or.11.2.289
  • Pages: 289-292
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Abstract

We have investigated the effect of lupeol (lup-20(29)-ene-3β-ene-3-ol) isolated from mokumen (Gossampinus malabarica L. Merr) on DNA of human promyelotic HL-60 leukemia cells. Induction of apoptosis by lupeol was observed in human leukemia HL-60 cells. Morphological change showing apoptotic bodies was observed in the HL-60 cells treated with lupeol. The fragmentation of DNA by lupeol to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the HL-60 cells. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 28.5, 42.0 and 70.9% after a 3-day treatment with 75, 100 and 150 µM lupeol, respectively. The data of the present study show that the suppression by lupeol of growth of the HL-60 cells results from the induction of apoptosis by this compound.

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February 2004
Volume 11 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Aratanechemuge Y, Hibasami H, Sanpin K, Katsuzaki H, Imai K and Komiya T: Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells. Oncol Rep 11: 289-292, 2004.
APA
Aratanechemuge, Y., Hibasami, H., Sanpin, K., Katsuzaki, H., Imai, K., & Komiya, T. (2004). Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells. Oncology Reports, 11, 289-292. https://doi.org/10.3892/or.11.2.289
MLA
Aratanechemuge, Y., Hibasami, H., Sanpin, K., Katsuzaki, H., Imai, K., Komiya, T."Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells". Oncology Reports 11.2 (2004): 289-292.
Chicago
Aratanechemuge, Y., Hibasami, H., Sanpin, K., Katsuzaki, H., Imai, K., Komiya, T."Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells". Oncology Reports 11, no. 2 (2004): 289-292. https://doi.org/10.3892/or.11.2.289