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Expression and promoter methylation analysis of ATP-binding cassette genes in pancreatic cancer

  • Authors:
    • Minyong Chen
    • Xiaofeng Xue
    • Feitong Wang
    • Yong An
    • Dong Tang
    • Yue Xu
    • Hui Wang
    • Zhongxu Yuan
    • Wentao Gao
    • Jishu Wei
    • Jingjing Zhang
    • Yi Miao

  • View Affiliations

  • Published online on: September 28, 2011     https://doi.org/10.3892/or.2011.1475
  • Pages: 265-269
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Abstract

We investigated the relationship of ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP expression and promoter methylation with pancreatic cancer tumorigenesis and drug resistance. Gemcitabine-resistant pancreatic cancer cells, SW1990/GZ (33.3-fold increased resistance), were obtained by treating SW1990 cells with gemcitabine. The expression of ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP was determined by quantitative real-time RT-PCR in the cell lines, 3 normal pancreatic tissues, 15 human pancreatic cancer samples and 15 adjacent tissues. Promoter methylation was determined in cell lines by bisulfite genomic sequencing. ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP were upregulated in SW1990 and SW1990/GZ compared with normal pancreatic tissue, and expression in SW1990/GZ was significantly higher than in SW1990 cells. ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP were upregulated in pancreatic cancer tissues, compared to adjacent tissues. The ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP promoter were hypomethylated in all the cell lines. ABCB1/MDR1, ABCC1/MRP1 and ABCG2/BCRP expression correlated with pancreatic cancer tumorigenesis and drug resistance in a mechanism that is independent of promoter methylation.

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January 2012
Volume 27 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen M, Xue X, Wang F, An Y, Tang D, Xu Y, Wang H, Yuan Z, Gao W, Wei J, Wei J, et al: Expression and promoter methylation analysis of ATP-binding cassette genes in pancreatic cancer. Oncol Rep 27: 265-269, 2012.
APA
Chen, M., Xue, X., Wang, F., An, Y., Tang, D., Xu, Y. ... Miao, Y. (2012). Expression and promoter methylation analysis of ATP-binding cassette genes in pancreatic cancer. Oncology Reports, 27, 265-269. https://doi.org/10.3892/or.2011.1475
MLA
Chen, M., Xue, X., Wang, F., An, Y., Tang, D., Xu, Y., Wang, H., Yuan, Z., Gao, W., Wei, J., Zhang, J., Miao, Y."Expression and promoter methylation analysis of ATP-binding cassette genes in pancreatic cancer". Oncology Reports 27.1 (2012): 265-269.
Chicago
Chen, M., Xue, X., Wang, F., An, Y., Tang, D., Xu, Y., Wang, H., Yuan, Z., Gao, W., Wei, J., Zhang, J., Miao, Y."Expression and promoter methylation analysis of ATP-binding cassette genes in pancreatic cancer". Oncology Reports 27, no. 1 (2012): 265-269. https://doi.org/10.3892/or.2011.1475