miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation

  • Authors:
    • Benjamin Vicinus
    • Claudia Rubie
    • Nathalie Stegmaier
    • Vilma Oliveira Frick
    • Kathrin Kölsch
    • Anne Kauffels
    • Pirus Ghadjar
    • Mathias  Wagner
    • Matthias Glanemann
  • View Affiliations

  • Published online on: July 1, 2013     https://doi.org/10.3892/or.2013.2580
  • Pages: 1285-1292
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recently, we reported a functional interaction between miR-21 and its identified chemokine target CCL20 in colorectal cancer (CRC) cell lines. Here, we investigated whether such functional interactions are permitted at the cellular level which would require an inverse correlation of expression and also co-expression of miR-21 and CCL20 in the same cell. Expression profiling was performed using qPCR, and ELISA, in situ hybridization and immunohistochemistry were applied for the presentation of their cellular localization. We demonstrated that miR-21 as well as CCL20 were both significantly upregulated in CRC tissues; thus, showing no antidromic expression pattern. This provided an initial clue that miR-21 and CCL20 may not be expressed in the same cell. In addition, we located miR-21 expression at the cellular level predominantly in stromal cells such as tumor-associated fibroblasts and to a minor degree in immune cells such as macrophages and lymphocytes. Likewise, CCL20 expression was primarily detected in tumor-infiltrating immune cells. Thus, investigating the cellular localization of miR-21 and its target CCL20 revealed that both molecules are expressed predominantly in the microenvironment of CRC tumors.

Related Articles

Journal Cover

September 2013
Volume 30 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Vicinus B, Rubie C, Stegmaier N, Frick VO, Kölsch K, Kauffels A, Ghadjar P, Wagner M and Glanemann M: miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation. Oncol Rep 30: 1285-1292, 2013.
APA
Vicinus, B., Rubie, C., Stegmaier, N., Frick, V.O., Kölsch, K., Kauffels, A. ... Glanemann, M. (2013). miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation. Oncology Reports, 30, 1285-1292. https://doi.org/10.3892/or.2013.2580
MLA
Vicinus, B., Rubie, C., Stegmaier, N., Frick, V. O., Kölsch, K., Kauffels, A., Ghadjar, P., Wagner, M., Glanemann, M."miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation". Oncology Reports 30.3 (2013): 1285-1292.
Chicago
Vicinus, B., Rubie, C., Stegmaier, N., Frick, V. O., Kölsch, K., Kauffels, A., Ghadjar, P., Wagner, M., Glanemann, M."miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation". Oncology Reports 30, no. 3 (2013): 1285-1292. https://doi.org/10.3892/or.2013.2580