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Genomic semi-automated cycle sequencing as a sensitive screening technique for p53 mutations in frozen tumor samples.

  • Authors:
    • S Wang-Gohrke
    • S Hees
    • A Pochon
    • W H Wen
    • A Reles
    • M F Press
    • R Kreienberg
    • I B Runnebaum

  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/or.5.1.65
  • Pages: 65-73
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Abstract

Single-strand conformational polymorphism (SSCP) is the most widely used method for p53 gene mutation screening. Nucleotide sequence analysis is considered more sensitive for detection of mutations. We established a genomic semi-automated cycle sequencing protocol suitable for p53 gene mutation screening. The technique was applied to 44 SSCP-negative frozen ovarian cancer samples: Eleven mutations (11/44, 25%) were found, 6 point missense mutations, 3 silent point mutations, 1 nonsense mutation and 1 single-base deletion. Heterozygous mutations were readily detectable. Genomic semi-automated cycle sequencing is a sensitive, time-effective screening method requiring only small amounts of tumor tissue.

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Jan-Feb 1998
Volume 5 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
  • Ranked Oncology
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Wang-Gohrke S, Hees S, Pochon A, Wen W, Reles A, Press M, Kreienberg R and Runnebaum I: Genomic semi-automated cycle sequencing as a sensitive screening technique for p53 mutations in frozen tumor samples.. Oncol Rep 5: 65-73, 1998.
APA
Wang-Gohrke, S., Hees, S., Pochon, A., Wen, W., Reles, A., Press, M. ... Runnebaum, I. (1998). Genomic semi-automated cycle sequencing as a sensitive screening technique for p53 mutations in frozen tumor samples.. Oncology Reports, 5, 65-73. https://doi.org/10.3892/or.5.1.65
MLA
Wang-Gohrke, S., Hees, S., Pochon, A., Wen, W., Reles, A., Press, M., Kreienberg, R., Runnebaum, I."Genomic semi-automated cycle sequencing as a sensitive screening technique for p53 mutations in frozen tumor samples.". Oncology Reports 5.1 (1998): 65-73.
Chicago
Wang-Gohrke, S., Hees, S., Pochon, A., Wen, W., Reles, A., Press, M., Kreienberg, R., Runnebaum, I."Genomic semi-automated cycle sequencing as a sensitive screening technique for p53 mutations in frozen tumor samples.". Oncology Reports 5, no. 1 (1998): 65-73. https://doi.org/10.3892/or.5.1.65