Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro
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- Published online on: May 1, 2001 https://doi.org/10.3892/or.8.3.615
- Pages: 615-619
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Abstract
We investigated whether or not the mitochondrial genotypes affect radiation-induced micronucleus (MN) formation. For that purpose, the ρ+, KT1 and ρ0 human osteosarcoma cell lines were used, which carry the wild-type mitochondrial DNA (mtDNA), the tRNALys mutant mtDNA and no mtDNA, respectively. Despite no significant difference in the clonogenic radiosensitivity, the ρ+, KT1 and ρ0 cells exhibited high, intermediate and low radiosensitivities, respectively, to the MN induction in cytokinesis-blocked binucleated cells. Such differential MN inductions were correlated with high, intermediate and low levels of cellular ATP in the ρ+, KT1 and ρ0 cells, respectively, but not exactly with ROS production. Antimycin A that inhibits the respiratory complex III reduced the rate of radiation-induced MN induction in the ρ+ and KT1, but not ρ0 cells. Thus, the functional status of the mtDNA to produce ATP appears to play a significant role for radiation-induced MN.