Successive cultures of mature hepatocytes for hepatocyte autotransplantation to assist liver function after liver resection for cancer

Funaki,N.; Tanaka,J.; Sugiyama,T.; Ohshio,G.; Nonaka,A.; Yotsumoto,F.; Sugie,T.; Imamura,M.

doi:10.3892/or.9.4.713

Successive cultures of mature hepatocytes for hepatocyte autotransplantation to assist liver function after liver resection for cancer

Authors:
- N. Funaki
- J. Tanaka
- T. Sugiyama
- G. Ohshio
- A. Nonaka
- F. Yotsumoto
- T. Sugie
- M. Imamura
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Affiliations: Department of Surgery, Shiga Medical Center for Adults, Moriyama City, Shiga 524, Japan
Published online on: July 1, 2002 https://doi.org/10.3892/or.9.4.713
Pages: 713-721

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Abstract

We developed a method for the rapid successive cultures of adult rat mature hepatocytes on plastic dishes while avoiding viral transformation or co-culture with other cell lines. This method also allows for culturing adult human mature hepatocytes up to the secondary culture. These can be expected to provide a good source for hepatocyte autotransplantation, and, combined with the previously reported methods for the transplantation of hepatocytes into the spleen, a promising option for the support of liver function after liver resection for cancer without the need for immunosuppressive agents.